Hood Robert B, Liang Donghai, Tang Ziyin, Kloog Itai, Schwartz Joel, Laden Francine, Jones Dean, Gaskins Audrey J
Department of Epidemiology, Emory University Rollins School of Public Health, Atlanta, Georgia, USA.
Department of Environmental Health, Emory University Rollins School of Public Health, Atlanta, Georgia, USA.
Environ Epidemiol. 2022 Feb 4;6(1):e191. doi: 10.1097/EE9.0000000000000191. eCollection 2022 Feb.
Both acute and chronic exposure to fine particulate matter (PM) have been linked to negative health outcomes. Studies have used metabolomics to describe the biological pathways linking PM with disease but have focused on a single exposure window. We compared alterations in the serum metabolome following various short- and long-term PM exposures.
Participants were women undergoing fertilization at a New England fertility clinic (n = 200). Women provided their residential address and provided a blood sample during controlled ovarian stimulation. PM exposure was estimated in the 1, 2, and 3 days, 2 weeks, and 3 months prior to blood collection using a validated spatiotemporal model. We utilized liquid chromatography with high-resolution mass spectrometry. We used generalized linear models to test for associations between metabolomic features and PM exposures after adjusting for potential confounders. Significant features ( < 0.005) were used for pathway analysis and metabolite identification.
We identified 17 pathways related to amino acid, lipid, energy, and nutrient metabolism that were solely associated with acute PM exposure. Fifteen pathways, mostly, pro-inflammatory, anti-inflammatory, amino acid, and energy metabolism, were solely associated with long-term PM exposure. Seven pathways were associated with the majority of exposure windows and were mostly related to anti-inflammatory and lipid metabolism. Among the significant features, we confirmed seven metabolites with level-1 evidence.
We identified serum metabolites and metabolic pathways uniquely associated with acute versus chronic PM exposure. These different biologic pathways may help explain differences in disease states when investigating different lengths of PM exposure.
急性和慢性暴露于细颗粒物（PM）均与负面健康结果相关。研究已使用代谢组学来描述将PM与疾病联系起来的生物学途径，但这些研究都集中在单一暴露窗口。我们比较了在各种短期和长期PM暴露后血清代谢组的变化。
参与者为在新英格兰一家生育诊所接受受精的女性（n = 200）。女性提供其居住地址，并在控制性卵巢刺激期间提供血样。使用经过验证的时空模型估算采血前1天、2天和3天、2周以及3个月的PM暴露情况。我们采用了液相色谱与高分辨率质谱联用技术。在调整潜在混杂因素后，我们使用广义线性模型来检验代谢组学特征与PM暴露之间的关联。显著特征（< 0.005）用于通路分析和代谢物鉴定。
我们确定了17条与氨基酸、脂质、能量和营养代谢相关的通路，这些通路仅与急性PM暴露相关。15条通路，主要是促炎、抗炎、氨基酸和能量代谢通路，仅与长期PM暴露相关。7条通路与大多数暴露窗口相关，且大多与抗炎和脂质代谢有关。在显著特征中，我们以一级证据确认了7种代谢物。
我们确定了与急性和慢性PM暴露独特相关的血清代谢物和代谢通路。在研究不同时长的PM暴露时，这些不同的生物学通路可能有助于解释疾病状态的差异。
