Beese L, Feder R, Sayre D
Biophys J. 1986 Jan;49(1):259-68. doi: 10.1016/S0006-3495(86)83639-6.
Contact x-ray microscopy potentially allows living, wet cells to be visualized at a resolution of up to 100 A. Furthermore, differential absorption by specific elements permits the study of the distribution of those elements in biological specimens. In contact x-ray microscopy, soft x-rays (10 A to 100 A) pass through a biological sample and expose an underlying x-ray sensitive polymer (resist), producing an image that reflects the photon absorbance within the specimen. The high penetrating power of soft x-ray enables images to be obtained from specimens up to several microns thick. In this paper, the technique is described, some of the areas currently under study are considered, and biological examples of the use of contact x-ray microscopy are given.
接触式X射线显微镜有可能使活的湿细胞以高达100埃的分辨率成像。此外，特定元素的差分吸收允许研究这些元素在生物标本中的分布。在接触式X射线显微镜中，软X射线（10埃至100埃）穿过生物样品并使下面的X射线敏感聚合物（抗蚀剂）曝光，产生反映标本内光子吸收率的图像。软X射线的高穿透能力使我们能够从厚度达几微米的标本中获取图像。本文描述了该技术，考虑了一些当前正在研究的领域，并给出了接触式X射线显微镜应用的生物学实例。
