Papadatos Stamatis S, Mitselou Antigoni, Lampri Evangeli, Varouktsi Anna, Grammeniatis Vasileios, Klaroudas Antonios, Katsanos Konstantinos, Galani Vasiliki
Department of Anatomy-Histology-Embryology, University of Ioannina, Ioannina, Greece.
Internal Medicine Department, Spiliopoulio - "Aghia Eleni" Hospital of Athens, Athens, Greece.
Maedica (Bucur). 2024 Sep;19(3):478-485. doi: 10.26574/maedica.2024.19.3.478.
BACKGROUND/AIM: This study aims to explore the role of NF-kB p65/p50 expression in patients with inflammatory bowel disease (IBD), those with irritable bowel syndrome (IBS) and normal controls.
Twenty patients with IBS, 19 subjects with IBD and 18 normal controls, who underwent colonoscopy, provided us with biopsies from the terminal ileum, cecum and rectum. Patients with IBS were categorized according to bowel habits. Two polyclonal antibodies, alpha-p65 rabbit Ab and alpha-p50 rabbit Ab, were applied for immunohistochemical examination.
It was found that in patients with IBD, the expression of intensity and distribution of NF-kB p65/p50 were lower than in IBS patients and normal controls (p>0.05), but the NF-kB p65/p50 ratio was elevated (p>0.05). The NF-kB p65/p50 ratio was lower than in IBD patients and rather similar to normal controls. The distribution of NF-kB p65 as well as the distribution and intensity of NF-kB p50 expression were higher in IBS patients with predominant diarrhea, and the difference was statistically significant in the cecum epithelium. The NF-kB p65/p50 expression ratio was higher in IBS patients with predominant diarrhea, but statistical significance was documented only in cecum epithelium specimens.
The NF-kB p65/p50 ratio offers more significant information than each subunit. The increased expression of NF-kB p50 in IBS patients compared to IBD subjects or controls seems to be an early event in the process of inflammation. The similar pattern of NF-kB p65/p50 expression in IBS patients with predominant diarrhea and IBD patients may suggest possible common pathogenetic pathways.
背景/目的：本研究旨在探讨核因子-κB p65/p50表达在炎症性肠病（IBD）患者、肠易激综合征（IBS）患者及正常对照中的作用。
20例IBS患者、19例IBD患者及18名正常对照者接受了结肠镜检查，并提供了来自回肠末端、盲肠和直肠的活检组织。IBS患者根据排便习惯进行分类。应用两种多克隆抗体，α-p65兔抗体和α-p50兔抗体进行免疫组织化学检查。
发现IBD患者中，核因子-κB p65/p50的表达强度和分布低于IBS患者和正常对照（p>0.05），但核因子-κB p65/p50的比值升高（p>0.05）。IBS患者的核因子-κB p65/p50比值低于IBD患者，且与正常对照相当。以腹泻为主的IBS患者中，核因子-κB p65的分布以及核因子-κB p50表达的分布和强度较高，在盲肠上皮中差异具有统计学意义。以腹泻为主的IBS患者中核因子-κB p65/p50的表达比值较高，但仅在盲肠上皮标本中具有统计学意义。
核因子-κB p65/p50的比值比每个亚基提供了更有意义的信息。与IBD患者或对照相比，IBS患者中核因子-κB p50表达增加似乎是炎症过程中的早期事件。以腹泻为主的IBS患者和IBD患者中核因子-κB p65/p50的表达模式相似，这可能提示存在共同的发病机制途径。
