Zhou Ruoyu, Bai Guangfu, Zhu Dongxu, Xu Qiong, Zhang Xudong, Li Tianran, Qian Yayun, Bu Chiwen
Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225001, China.
Affiliated Huishan Hospital of Medical College, Yangzhou University, Wuxi Huishan District People's Hospital, Wuxi 214187, Jiangsu Province, China.
Biomed Opt Express. 2024 Oct 24;15(11):6469-6485. doi: 10.1364/BOE.542523. eCollection 2024 Nov 1.
In this study, we present a pump-free SERS microfluidic chip capable of detecting liver cancer-related miR-21 and miR-155 concurrently with ultra-sensitivity and high efficiency. We employed a FeO@cDNA-AuNPs@Raman reporter@H composite structure and a recognition competition strategy. When the target miRNAs (miR-21 and miR-155) are present in the test liquid, they specifically compete with the nucleic acid complementary strand(H) of FeO@cDNA-AuNPs@Raman reporter@H, causing AuNPs to competitively detach from the surface of FeO, resulting in a decrease in the SERS signal. Consequently, this pump-free SERS microfluidic chip enables the detection of the target miRNAs more rapidly and accurately in complex environments. This method offers an approach for the simultaneous and efficient detection of miRNAs and holds promising applications in the early diagnosis of liver cancer.
在本研究中，我们展示了一种无泵表面增强拉曼散射（SERS）微流控芯片，它能够以超灵敏度和高效率同时检测与肝癌相关的miR-21和miR-155。我们采用了FeO@cDNA-AuNPs@拉曼报告分子@H复合结构和识别竞争策略。当测试液中存在目标微小核糖核酸（miRNAs，即miR-21和miR-155）时，它们会与FeO@cDNA-AuNPs@拉曼报告分子@H的核酸互补链（H）特异性竞争，导致金纳米粒子（AuNPs）竞争性地从FeO表面脱离，从而使SERS信号减弱。因此，这种无泵SERS微流控芯片能够在复杂环境中更快速、准确地检测目标miRNAs。该方法为miRNAs的同时高效检测提供了一种途径，在肝癌早期诊断中具有广阔的应用前景。
