Narawane Amit, Trout Robert, Viehland Christian, Kuo Anthony N, Vajzovic Lejla, Dhalla Al-Hafeez, Toth Cynthia A
bioRxiv. 2024 Oct 29:2024.10.25.620343. doi: 10.1101/2024.10.25.620343.
Suprachoroidal injections are a relatively new method of drug delivery to treat retinal disease. At present, it is difficult to visualize the distribution of injection-delivered product beneath the sclera into the suprachoroidal space. Imaging the suprachoroidal space with OCT is hindered by scattering of light from densely packed collagen fibers of the sclera, limiting depth penetration of the OCT light. In this Letter, we demonstrate the first use of tartrazine (Yellow 5) as an optical clearing agent for suprachoroidal research imaging with OCT in an eye model. Our results show that this clearing agent dramatically improves visualization of the choroid and suprachoroidal space with transscleral OCT and allows for improved imaging of the location and extent of delivered suprachoroidal fluid. We believe these methods will enable use of optical techniques such as OCT to image through a variety of previously optically inaccessible, highly scattering tissue samples.
脉络膜上腔注射是一种相对较新的用于治疗视网膜疾病的药物递送方法。目前，很难可视化注射递送的药物在巩膜下方进入脉络膜上腔的分布情况。用光学相干断层扫描（OCT）对脉络膜上腔进行成像受到巩膜密集排列的胶原纤维散射光的阻碍，限制了OCT光的深度穿透。在本信函中，我们展示了首次将酒石黄（黄色5号）用作光学清除剂，用于在眼部模型中通过OCT进行脉络膜上腔研究成像。我们的结果表明，这种清除剂通过经巩膜OCT显著改善了脉络膜和脉络膜上腔的可视化，并能更好地成像递送的脉络膜上腔液体的位置和范围。我们相信这些方法将使诸如OCT之类的光学技术能够对各种以前光学上无法进入的、高度散射的组织样本进行成像。
