Beilankouhi Shiva, Pourfarzad Amir, Ghanbarzadeh Babak, Rasouli Mousa, Hamishekar Hamed
Department of Pharmaceutical Sciences, Drug Applied Research Center University of Medical Sciences Tabriz Iran.
Department of Environmental Science Research Institute for Grapes and Raisin (RIGR) Hamedan Iran.
Food Sci Nutr. 2024 Jul 9;12(9):6789-6798. doi: 10.1002/fsn3.4330. eCollection 2024 Sep.
The utilization of grape stems, a by-product of the grape processing industry, as a source of valuable bioactive compounds, particularly polyphenols, has gained attention in recent years. This study aimed to investigate different eco-friendly extraction methods for obtaining polyphenols from grape ( cv. Bidaneh Sefid) stems, focusing on green solvents and innovative techniques. Four extraction methods were tested, involving the use of water and polyethylene glycol (PEG) as green solvents, along with maceration, microwave, ultrasound, and reduced-pressure techniques. High-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) was used to characterize and quantify the bioactive compounds in the extracts. A total of 29 polyphenols, including phenolic acids, flavonoids, proanthocyanidins, and stilbenes, were detected. Among the four extraction methods tested, methods 1 (water + microwave + ultrasound + atmospheric pressure) and 2 (water + microwave + ultrasound + reduced pressure) were found to be the most effective. Our study demonstrated that using water and PEG as green solvents, combined with techniques like microwave, ultrasound, and reduced pressure, effectively extracted both hydrophobic and hydrophilic compounds from the grape stems. These findings suggest that further exploration of these methods could lead to the development of value-added products from grape stems, emphasizing the significance of green extraction techniques for the recovery of polyphenols from winemaking by-products.
葡萄加工行业的副产品葡萄茎，作为有价值的生物活性化合物特别是多酚的来源，近年来受到了关注。本研究旨在探究从葡萄（品种Bidaneh Sefid）茎中获取多酚的不同环保提取方法，重点关注绿色溶剂和创新技术。测试了四种提取方法，包括使用水和聚乙二醇（PEG）作为绿色溶剂，以及浸渍法、微波法、超声法和减压法。采用高效液相色谱-电喷雾电离串联质谱联用（HPLC-ESI-MS/MS）对提取物中的生物活性化合物进行表征和定量。共检测到29种多酚，包括酚酸、黄酮类化合物、原花青素和芪类化合物。在所测试的四种提取方法中，方法1（水+微波+超声+常压）和方法2（水+微波+超声+减压）被发现是最有效的。我们的研究表明，使用水和PEG作为绿色溶剂，结合微波、超声和减压等技术，能有效地从葡萄茎中提取疏水性和亲水性化合物。这些发现表明，进一步探索这些方法可能会开发出葡萄茎的增值产品，强调了绿色提取技术对于从酿酒副产品中回收多酚的重要性。
