Wu Xiaofan, He Yupo, Fu Jiaqi, Zhao Yanli
College of Chemistry and Pharmacy, Jiamusi University, Jiamusi 154007, China.
Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
ACS Omega. 2024 Oct 29;9(45):44907-44915. doi: 10.1021/acsomega.4c00756. eCollection 2024 Nov 12.
Evaluating the bacterial activity effectively is critical to addressing the challenges posed by bacterial infections. Electrochemistry offers significant advantages in accuracy and cost efficiency compared with methods that are more time-consuming or require expensive instrumentation. This study initially established an electrochemical method for detecting bacterial activity using heat treatment as the pretreatment step. Subsequent optimization of the heat treatment and detection conditions further enhanced the method efficiency. The detection results of () and () under different heating conditions showed that the peak current values of and were the highest after heating at 50 °C for 15 min. The dynamic changes in the electrochemical signals from the heat-treated bacteria with alteration in heating conditions were indicated to be related to the effects of heating on the external structure and purine metabolism of the bacterial cells. Although heat-treated and exhibited similar peak potentials, different substances were observed to have contributed to these potentials. A positive linear correlation was observed between the peak current and bacterial concentration. Compared to the plate counting method, the electrochemical method detected the activity changes in and from the logarithmic to the stationary phase earlier. The findings support the broad application of electrochemical methods in microbial detection and provide valuable insights into the purine metabolism of heat-stressed bacteria.
有效评估细菌活性对于应对细菌感染带来的挑战至关重要。与那些更耗时或需要昂贵仪器的方法相比，电化学在准确性和成本效益方面具有显著优势。本研究最初建立了一种以热处理作为预处理步骤来检测细菌活性的电化学方法。随后对热处理和检测条件的优化进一步提高了该方法的效率。在不同加热条件下对（）和（）的检测结果表明，在50℃加热15分钟后，和的峰值电流值最高。经热处理的细菌的电化学信号随加热条件变化的动态变化表明，这与加热对细菌细胞外部结构和嘌呤代谢的影响有关。尽管经热处理的和表现出相似的峰值电位，但观察到不同物质对这些电位有贡献。峰值电流与细菌浓度之间呈正线性相关。与平板计数法相比，电化学方法能更早检测到和从对数期到稳定期的活性变化。这些发现支持了电化学方法在微生物检测中的广泛应用，并为热应激细菌的嘌呤代谢提供了有价值的见解。
