Institute of Chinese Medicine Resources, Shandong Academy of Chinese Medicine, Jinan 250014, PR China; Shandong Yate Ecological Technology Co., Ltd., Linyi 276017, PR China; State Key Lab of Microbial Technology, Shandong University, Qingdao 266237, PR China.
Institute of Chinese Medicine Resources, Shandong Academy of Chinese Medicine, Jinan 250014, PR China.
Gene. 2023 Dec 20;888:147739. doi: 10.1016/j.gene.2023.147739. Epub 2023 Aug 24.
The active ingredients of many medicinal plants are the secondary metabolites associated with the growth period. Lonicera japonica Thunb. is an important traditional Chinese medicine, and the flower development stage is an important factor that influences the quality of medicinal ingredients. In this study, transcriptomics and metabolomics were performed to reveal the regulatory mechanism of secondary metabolites during flowering of L. japonica. The results showed that the content of chlorogenic acid (CGA) and luteolin gradually decreased from green bud stage (Sa) to white flower stage (Sc), especially from white flower bud stage (Sb) to Sc. Most of the genes encoding the crucial rate-limiting enzymes, including PAL, C4H, HCT, C3'H, F3'H and FNSII, were down-regulated in three comparisons. Correlation analysis identified some members of the MYB, AP2/ERF, bHLH and NAC transcription factor families that are closely related to CGA and luteolin biosynthesis. Furthermore, differentially expressed genes (DEGs) involved in hormone biosynthesis, signalling pathways and flowering process were analysed in three flower developmental stage.
许多药用植物的活性成分是与生长周期相关的次生代谢物。忍冬是一种重要的传统中药，其花期是影响药用成分质量的重要因素。本研究采用转录组学和代谢组学方法揭示了忍冬开花过程中次生代谢物的调控机制。结果表明，绿原酸（CGA）和木樨草素的含量从绿芽期（Sa）逐渐下降到白花期（Sc），尤其是从白花蕾期（Sb）到 Sc。编码关键限速酶的大多数基因，包括 PAL、C4H、HCT、C3'H、F3'H 和 FNSII，在三个比较中均下调。相关性分析鉴定出与 CGA 和木樨草素生物合成密切相关的 MYB、AP2/ERF、bHLH 和 NAC 转录因子家族的一些成员。此外，还分析了三个花发育阶段中与激素生物合成、信号通路和开花过程相关的差异表达基因（DEGs）。
