Synthesis of nanohybrid consisting of taurine derived carbon dots and nanoceria for anticancer applications.

Here, we first synthesized carbon dots (CDs) from taurine and then a nanohybrid with ceria (CeO) nanoparticles using thermal decomposition method for checking their antineoplastic efficacy against human triple negative mammary carcinoma cells, MDA-MB-231. The in vitro study demonstrated significant dose-dependent antineoplastic activity of the nanohybrids within a range of concentration from 10 to 50 μg/mL after 48 h of treatment. The cellular morphology analysis clearly depicted substantial amount of cell death which seems to stem from increased intracellular reactive oxygen species (ROS) production. However, the maximum anticancer activity of the nanohybrid as compared to bare CDs and CeO is supposed to be due to the combined anticancer effect of both CDs and CeO (a well-established antitumor agent). Further we have performed molecular docking study to reveal the anticancer mechanism of CDs which exhibited high binding capacity towards several proapoptotic and antiapoptotic protein molecules. The binding affinity values were found to be - 8.7 kcal/mol, - 7.9 kcal/mol, - 9.6 kcal/mol, - 9.5 kcal/mol, - 12 kcal/mol and - 11.1 kcal/mol for BAD, BCl-2, p53, Caspase-8, Caspase-9 and Caspase-3 respectively. Taken together, our synthesized CDs-CeO nanohybrid could be thought as a potential anticarcinogenic option in the field of breast cancer therapeutics.