Zhou Ruigang, Huang Junjie, Zhang Wenhai, Wang Weimei, Peng Weilong, Chen Jun, Yu Chenglong, Bo Ruonan, Liu Mingjiang, Li Jingui
School of Veterinary Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, PR China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China.
Mater Today Bio. 2024 Oct 30;29:101315. doi: 10.1016/j.mtbio.2024.101315. eCollection 2024 Dec.
Bacterial-infected wounds could cause delayed wound healing due to increased inflammation, especially wounds infected by drug-resistant bacteria remain a major clinical problem. However, traditional treatment strategies were gradually losing efficacy, such as the abuse of antibiotics leading to enhanced bacterial resistance. Therefore, there was an urgent need to develop an antibiotic-free multifunctional dressing for bacterially infected wound healing. This study demonstrated the preparation of a multifunctional injectable hydrogel and evaluated its efficacy in treating acute and infected wounds. The hydrogel was prepared by a one-step mixing method, and cross-linked by natural deep eutectic solvent (DES), polyvinyl alcohol (PVA), chitosan (CS), tannic acid (TA), and Cu through non-covalent interactions (hydrogen bonds and metal coordination bonds). PVA/CS/DES/CuTA hydrogel has multiple functional properties, including injectability, tissue adhesion, biocompatibility, hemostasis, broad-spectrum antibacterial, anti-inflammatory, and angiogenesis. Most importantly, in the -infected skin wound model, PVA/CS/DES/CuTA hydrogel could ultimately accelerate infected wound healing by killing bacteria, activating M2 polarization, inhibiting inflammation, and promoting angiogenesis. In summary, the PVA/CS/DES/CuTA hydrogel showed great potential as a wound dressing for bacterial infected wounds treatment in the clinic.
细菌感染的伤口会因炎症加剧而导致伤口愈合延迟，尤其是耐药菌感染的伤口仍是一个主要的临床问题。然而，传统的治疗策略正逐渐失去疗效，比如抗生素的滥用导致细菌耐药性增强。因此，迫切需要开发一种用于细菌感染伤口愈合的无抗生素多功能敷料。本研究展示了一种多功能可注射水凝胶的制备，并评估了其在治疗急性和感染伤口方面的疗效。该水凝胶通过一步混合法制备，并通过天然低共熔溶剂（DES）、聚乙烯醇（PVA）、壳聚糖（CS）、单宁酸（TA）和铜通过非共价相互作用（氢键和金属配位键）交联而成。PVA/CS/DES/CuTA水凝胶具有多种功能特性，包括可注射性、组织粘附性、生物相容性、止血、广谱抗菌、抗炎和血管生成。最重要的是，在感染性皮肤伤口模型中，PVA/CS/DES/CuTA水凝胶最终可通过杀灭细菌、激活M2极化、抑制炎症和促进血管生成来加速感染伤口的愈合。综上所述，PVA/CS/DES/CuTA水凝胶在临床上作为细菌感染伤口治疗的伤口敷料显示出巨大潜力。
