Shar Saw Bo Day, Nguyen Cuong Dinh, Sanada-Morimura Sachiyo, Zheng Shao-Hui, Fujita Daisuke
The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Loikaw Research Center, Department of Agricultural Research, Loikaw 09011, Kayah State, Myanmar.
Breed Sci. 2024 Jun;74(3):183-192. doi: 10.1270/jsbbs.23066. Epub 2024 Jul 2.
The brown planthopper (BPH; Stål) is a devastating pest that causes severe rice yield losses in Asia. Introducing multiple BPH resistance genes into rice cultivars is an effective and sustainable way to mitigate yield losses. A traditional rice cultivar, 'Rathu Heenati', has durable BPH resistance due to multiple resistance genes (including and ) and quantitative trait loci (QTLs). However, these genes have not been used in Japanese rice breeding owing to limited genetic information. To identify markers tightly linked to and introgressed into the 'Sagabiyori' (susceptible) genetic background, we performed substitution mapping. was delimited between RM3132 and RM589 on chromosome 6, and between RM16493 and RM16531 on chromosome 4. We also performed QTL analysis to identify additional BPH resistance genes from 'Rathu Heenati' and detected a QTL, denoted as , on chromosome 3. The effect of pyramiding and was significantly greater against virulent BPH populations than that of either gene alone. The combination of , and from 'Rathu Heenati' might be facilitated to improve commercial Japanese cultivars with more robust BPH resistance.
褐飞虱（BPH；Stål）是一种极具破坏力的害虫，在亚洲导致水稻产量严重损失。将多个抗褐飞虱基因导入水稻品种是减轻产量损失的有效且可持续的方法。一个传统水稻品种“Rathu Heenati”由于多个抗性基因（包括 和 ）以及数量性状位点（QTL）而具有持久的抗褐飞虱能力。然而，由于遗传信息有限，这些基因尚未在日本水稻育种中得到应用。为了鉴定与导入“佐贺美百合”（感虫）遗传背景中的 和 紧密连锁的标记，我们进行了代换作图。 将区间定位在第6染色体上的RM3132和RM589之间， 将区间定位在第4染色体上的RM16493和RM16531之间。我们还进行了QTL分析，以从“Rathu Heenati”中鉴定其他抗褐飞虱基因，并在第3染色体上检测到一个QTL，记为 。将 和 聚合在一起对毒性褐飞虱种群的效果显著大于单独使用任何一个基因的效果。导入“Rathu Heenati”中的 、 和 的组合可能有助于培育出对褐飞虱具有更强抗性的日本商业品种。
