1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi University, Nanning, 530005 China.
2Maize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007 China.
Hereditas. 2019 Jun 26;156:22. doi: 10.1186/s41065-019-0098-4. eCollection 2019.
Rice planthoppers (main brown planthopper, Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions. Notably, host-plant resistance was proved to be one of the effective ways to manage the pest. And stronger virulence will probably emergence when continuous use of insecticides. Therefore, more resistance genes with different resistance mechanisms were needed to be detected and then applied in the rice breeding practice.
Resistance genes in the rice variety IR64 were evaluated considering the seedling bulk test and seedling survival rate. As a result, a locus with a large LOD score of 7.23 was found between markers RM302 and YM35 on chromosome 1. The locus explained 36.9% of phenotypic variation and was tentatively denominated . Moreover, was detected to be harbored by the markers RM28366 and RM463, and had the largest LOD score of 2.08, explaining 7.7% of phenotypic variance in the same mapping population. Finally, the preliminary-near-isogenic-lines (pre-NILs) carrying exhibited significant tolerance to the insects. But no antibiotic or antixenotic effects were observed in the resistant plants when infested with the insects.
We mapped one major BPH resistance gene in consideration of seedling survival rate and the resistance lines showed tolerance to BPH. The detected gene should be beneficial for understanding the resistance mechanism of rice to BPH and for insect-resistance rice breeding programs.
褐飞虱（主要是褐飞虱，Stål；BPH）是亚洲水稻生产的重大威胁之一，因为其在田间条件下具有严重的破坏性和难以控制。值得注意的是，寄主植物抗性被证明是管理这种害虫的有效方法之一。并且，当连续使用杀虫剂时，可能会出现更强的毒力。因此，需要检测更多具有不同抗性机制的抗性基因，并将其应用于水稻育种实践中。
考虑到苗批试验和苗存活率，评估了水稻品种 IR64 中的抗性基因。结果，在标记 RM302 和 YM35 之间的第 1 号染色体上发现了一个 LOD 得分高达 7.23 的基因座。该基因座解释了 36.9%的表型变异，并被暂定命名为 。此外，在相同的作图群体中，还检测到 被标记 RM28366 和 RM463 所携带，其 LOD 得分最大为 2.08，解释了 7.7%的表型方差。最后，携带 的初步近等基因系（pre-NILs）表现出对昆虫的显著耐受性。但是，在受昆虫侵害时，抗性植株没有观察到抗生素或抗生作用。
我们考虑到苗存活率，定位了一个主要的 BPH 抗性基因 ，抗性系对 BPH 表现出耐受性。检测到的基因应该有助于理解水稻对 BPH 的抗性机制，并有助于昆虫抗性水稻的育种计划。
