Gu Yixin, Zhan Yu, Guo Yafeng, Hua Wenyuan, Qi Xin, Gu Zhizhi, Cao Shengnan, Chen Yan, Xue Zhuang, Wang Wei
Key Laboratory of Applied Biology and Aquaculture of Northern Fishes in Liaoning Province, Dalian Ocean University, Dalian 116023, China.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Aquac Nutr. 2024 Apr 5;2024:5566739. doi: 10.1155/2024/5566739. eCollection 2024.
Cinnamaldehyde is an ideal feed additive with good immune enhancement and anti-inflammatory regulation effects. However, the anti-inflammatory regulation mechanism in fat greenling (, ) remains unclear. The nine targets of cinnamaldehyde were gathered in identified by the Traditional Chinese Medicine Systems Pharmacology database and Uniprot database, and 1,320 intestinal inflammation disease (IIF)-related proteins were screened from DrugBank, Online Mendelian Inheritance in Man (OMIM), Genecards, and Pharmacogenetics and Pharmacogenomics Knowledge Base (PHARMGKB) Databases. According to the Gene Ontology enrichment results and Kyoto Encyclopedia of Genes and Genomes pathway results, cinnamaldehyde may regulated the responses to bacteria, lipopolysaccharide, an inflammatory cytokine, and external stimuli via the nuclear factor kappa-B (NFB) signaling pathway within on inflammatory network. In addition, the protein-protein interaction analysis assisted in obtaining the closely related inflammatory regulatory proteins, including the C5a anaphylatoxin chemotactic receptor 1 (C5aR1), transcription factor p65 (RELA), prostaglandin G/H synthase 2 (PTGS2), and toll-like receptor 4 (TLR4), which were confirmed as the bottleneck nodes of the network to be more deeply verified via the molecular docking. Moreover, a cinnamaldehyde feeding model was established for evaluating the anti-inflammatory effect of cinnamaldehyde in . According to the current findings implied that cinnamaldehyde may play a protective role against IIF by reducing inflammation through the C5 complement (C5)/C5aR1/interleukin-6 (IL-6) and TLR4/NFB/PTGS2 pathway. The study focused on investigating the action mechanism of cinnamaldehyde on IIF through combining pharmacology and experimental verification in , which provided a fresh perspective on the promoting effect of cinnamaldehyde on IIF in fish.
肉桂醛是一种具有良好免疫增强和抗炎调节作用的理想饲料添加剂。然而，其在六线鱼体内的抗炎调节机制仍不清楚。通过中药系统药理学数据库和Uniprot数据库收集到肉桂醛的9个靶点，并从DrugBank、在线人类孟德尔遗传数据库（OMIM）、Genecards以及药物遗传学和药物基因组学知识库（PHARMGKB）数据库中筛选出1320种肠道炎症性疾病（IIF）相关蛋白。根据基因本体富集结果和京都基因与基因组百科全书通路结果，肉桂醛可能通过炎症网络中的核因子κB（NFB）信号通路调节对细菌、脂多糖、炎性细胞因子和外部刺激的反应。此外，蛋白质-蛋白质相互作用分析有助于获得密切相关的炎症调节蛋白，包括C5a过敏毒素趋化受体1（C5aR1）、转录因子p65（RELA）、前列腺素G/H合酶2（PTGS2）和Toll样受体4（TLR4），这些蛋白被确认为网络的瓶颈节点，将通过分子对接进行更深入的验证。此外，还建立了肉桂醛投喂模型以评估其在六线鱼体内的抗炎作用。根据目前的研究结果表明，肉桂醛可能通过C5补体（C5）/C5aR1/白细胞介素-6（IL-6）和TLR4/NFB/PTGS2途径减轻炎症，从而对IIF起到保护作用。该研究通过在六线鱼体内结合药理学和实验验证，重点研究了肉桂醛对IIF的作用机制，为肉桂醛对鱼类IIF的促进作用提供了新的视角。
