Wang Chenyang, Wei Di, Wei Zheng, Yang Dongxin, Xing Jing, Wang Yunze, Wang Xiaotong, Wang Pei, Ma Guannan, Zhang Xinru, Li Haolan, Tang Chuan, Hou Pengfei, Wang Jie, Gao Renjun, Xie Guiqiu, Li Cuidan, Ju Yingjiao, Wang Peihan, Yue Liya, Zhao Yongliang, Sheng Yongjie, Xiao Jingfa, Niu Haitao, Xu Sihong, Yang Huaiyi, Liu Di, Duan Bo, Bu Dongbo, Tan Guangming, Chen Fei
China National Center for Bioinformation, Beijing, 100101, China.
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China.
Adv Sci (Weinh). 2025 Mar;12(9):e2411354. doi: 10.1002/advs.202411354. Epub 2024 Nov 18.
In the face of exponential data growth, DNA-based storage offers a promising solution for preserving big data. However, most existing DNA storage methods, akin to traditional block printing, require costly chemical synthesis for each individual data file, adopting a sequential, one-time-use synthesis approach. To overcome these limitations, a novel, cost-effective "DNA-movable-type storage" system, inspired by movable type printing, is introduced. This system utilizes prefabricated DNA movable types-short, double-stranded DNA oligonucleotides encoding specific payload, address, and checksum data. These DNA-MTs are enzymatically ligated/assembled into cohesive sequences, termed "DNA movable type blocks," streamlining the assembly process with the automated BISHENG-1 DNA-MT inkjet printer. Using BISHENG-1, 43.7 KB of data files are successfully printed, assembled, stored, and accurately retrieved in diverse formats (text, image, audio, and video) in vitro and in vivo, using only 350 DNA-MTs. Notably, each DNA-MT, synthesized once (2 OD), can be used up to 10000 times, reducing costs to $122/MB-outperforming existing DNA storage methods. This innovation circumvents the need to synthesize entire DNA sequences encoding files from scratch, offering significant cost and efficiency advantages. Furthermore, it has considerable untapped potential to advance a robust DNA storage system, better meeting the extensive data storage demands of the big-data era.
面对呈指数级增长的数据，基于DNA的存储为保存大数据提供了一个很有前景的解决方案。然而，大多数现有的DNA存储方法类似于传统的活字印刷，对于每个单独的数据文件都需要进行昂贵的化学合成，采用的是顺序式、一次性使用的合成方法。为了克服这些局限性，一种受活字印刷启发的新颖且具有成本效益的“DNA活字存储”系统被引入。该系统利用预制的DNA活字——编码特定有效载荷、地址和校验和数据的短双链DNA寡核苷酸。这些DNA活字通过酶促连接/组装成连贯的序列，称为“DNA活字块”，利用自动化的毕昇-1 DNA活字喷墨打印机简化了组装过程。使用毕昇-1，仅使用350个DNA活字就成功地在体外和体内以多种格式（文本、图像、音频和视频）打印、组装、存储并准确检索了43.7 KB的数据文件。值得注意的是，每个DNA活字合成一次（2 OD）可使用多达10000次，将成本降低至122美元/MB，优于现有的DNA存储方法。这一创新避免了从头合成编码文件的整个DNA序列的需求，具有显著的成本和效率优势。此外，它在推进强大的DNA存储系统方面具有相当大的未开发潜力，能更好地满足大数据时代广泛的数据存储需求。
