Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University.
Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University;
J Vis Exp. 2024 Nov 1(213). doi: 10.3791/67254.
Currently, constructing a mouse model for cold environmental stimulation employs cold-heat plates and wearable cooling devices. These methods can partially fulfill the requirements for studying the responses and regulatory effects of mouse skin or neural circuits to cold stimulation. Numerous clinical studies have substantiated the correlation between exposure to low-temperature environments and the development of various diseases. Recently, there has been a growing emphasis on the continuous exchange of information between organs and tissues, providing a novel perspective on addressing longstanding issues within the human body. However, existing installations are unable to construct a model for mice inhaling cold air. Although placing mice in a cold environment seems attractive, it has considerable limitations. While mice inhale cold air, their skin is also being stimulated by the cold environment, making it unclear whether resulting pathological changes are due to lung stimulation through the interaction of distant organs or due to the skin receptors and neural signal transmission. This creates considerable confusion in related research. This scheme presents a new approach for constructing a mouse model for extreme cold air inhalation stimulation. This device allows mice to inhale extremely low-temperature gases while their bodies remain at a normal temperature. It maximizes the simulation of the stimulating effects of extreme ambient temperatures on mice and meets the research needs for studying the relationship between extreme environmental temperatures and related diseases.
目前，构建用于冷环境刺激的小鼠模型采用冷热板和可穿戴冷却装置。这些方法可以部分满足研究小鼠皮肤或神经回路对冷刺激的反应和调节作用的要求。许多临床研究证实了暴露于低温环境与各种疾病发展之间的相关性。最近，人们越来越关注器官和组织之间的信息持续交换，为解决人体内部的长期问题提供了新的视角。然而，现有的设备无法构建用于小鼠吸入冷空气的模型。尽管将小鼠置于冷环境中似乎很有吸引力，但它存在很大的局限性。当小鼠吸入冷空气时，它们的皮肤也会受到冷环境的刺激，因此不清楚由此产生的病理变化是由于通过远距离器官相互作用对肺部的刺激引起的，还是由于皮肤受体和神经信号传输引起的。这在相关研究中造成了相当大的混乱。本方案提出了一种构建用于极冷空气吸入刺激的小鼠模型的新方法。该装置允许小鼠在体温正常的情况下吸入极低温度的气体。它最大限度地模拟了极端环境温度对小鼠的刺激作用，满足了研究极端环境温度与相关疾病之间关系的研究需求。
