Bian Yu, Zhang Yuan, Ruan Ling-Yun, Feng Xue-Song
School of Pharmacy, China Medical University, Shenyang, China.
Crit Rev Anal Chem. 2024 Nov 18:1-19. doi: 10.1080/10408347.2024.2427128.
The physiological and officinal functions of phytosterols are of great significance, and recent dietary guidelines have underscored the significance of incorporating them into a balanced diet. Furthermore, it exhibits inhibitory effects on tumor growth, stimulates cellular immunity, possesses anti-inflammatory, antioxidant, and antidiabetic properties. To gain a more comprehensive understanding of the role of phytosterols in public health, it is crucial to establish simple, rapid, eco-conscious, efficient, and highly sensitive techniques for their extraction and determination across various matrices. This review presents a thorough overview of various techniques used for extracting and analyzing phytosterols in diverse plant-derived foods, encompassing a range of advanced technologies like solid-phase extraction, microextraction, supercritical fluid extraction, QuEChERS, alongside traditional approaches. The detection techniques include liquid chromatography-based methods, gas chromatography-based methods, supercritical fluid chromatography, and other methodologies. Additionally, we conduct a thorough examination and comparison of various techniques while proposing future prospects.
植物甾醇的生理和药用功能具有重要意义，近期的饮食指南强调了将其纳入均衡饮食的重要性。此外，它对肿瘤生长具有抑制作用，能刺激细胞免疫，具有抗炎、抗氧化和抗糖尿病特性。为了更全面地了解植物甾醇在公共卫生中的作用，建立简单、快速、环保、高效且高度灵敏的技术以在各种基质中提取和测定它们至关重要。本综述全面概述了用于提取和分析各种植物源食品中植物甾醇的各种技术，包括固相萃取、微萃取、超临界流体萃取、QuEChERS等一系列先进技术以及传统方法。检测技术包括基于液相色谱的方法、基于气相色谱的方法、超临界流体色谱法和其他方法。此外，我们对各种技术进行了全面审查和比较，并提出了未来展望。
