Whitson G L, Carrier W L, Francis A A, Shih C C, Georghiou S, Regan J D
Cell Tissue Kinet. 1986 Jan;19(1):39-47. doi: 10.1111/j.1365-2184.1986.tb00713.x.
A number of physical and chemical agents in the environment have been studied for their ability to induce or alter DNA repair mechanisms in human cells. We have investigated the effects of 60 Hz, 1000 V/cm electric fields on DNA repair in normal human fibroblasts in vitro. An examination was done on the ability of electric fields suspected to cause damage which could be repaired by thymine dimer excision and measurable by the bromodeoxyuridine photolysis assay. The thymine dimer assay with enzyme-sensitive site analysis was used to measure the cells' capacity for removing ultraviolet light (u.v.)-induced pyrimidine dimers; during exposure to electric field 24 hr before u.v. irradiation; 24 hr after u.v. irradiation; and up to 48 hr continuously after u.v. irradiation. Cell growth and cell survival following electric field exposure were also studied. Within the limits of these experiments, it was found that exposure to such electric fields did not alter cell growth or survival, and no DNA repair or alteration in DNA excision repair capacity was observed as compared with unexposed control cultures.
人们已经研究了环境中的多种物理和化学因子诱导或改变人类细胞中DNA修复机制的能力。我们已经在体外研究了60赫兹、1000伏/厘米的电场对正常人成纤维细胞中DNA修复的影响。对怀疑能造成可通过胸腺嘧啶二聚体切除修复并可用溴脱氧尿苷光解测定法测量的损伤的电场能力进行了检测。用酶敏感位点分析的胸腺嘧啶二聚体测定法来测量细胞去除紫外线（uv）诱导的嘧啶二聚体的能力；在紫外线照射前24小时暴露于电场期间；紫外线照射后24小时；以及紫外线照射后持续长达48小时。还研究了电场暴露后的细胞生长和细胞存活情况。在这些实验的范围内，发现暴露于这种电场不会改变细胞生长或存活，并且与未暴露的对照培养物相比，未观察到DNA修复或DNA切除修复能力的改变。
