Shiokawa K, Kawazoe Y, Tashiro K, Yamana K
Cell Differ. 1986 Mar;18(2):101-8. doi: 10.1016/0045-6039(86)90004-7.
Xenopus neurula cells were cultured in a medium that contained ammonium salts, amines, polyamines or alpha-methylornithine, and their rRNA synthesis was examined. All the ammonium salts and amines, but not polyamines, were strong and selective inhibitors of rRNA synthesis at 1.25-5.0 mM. alpha-Methylornithine did not inhibit rRNA synthesis, although it inhibited ornithine decarboxylase, an enzyme claimed to be a direct stimulator of rRNA synthesis. During the treatment ammonium ions and monomethylamines were accumulated within the treated cells. However, monomethylamines did not induce the accumulation of ammonium ions, and vice versa. Ammonium salts and amines also selectively inhibited rRNA synthesis in Xenopus borealis neurula cells.
将非洲爪蟾神经胚细胞培养在含有铵盐、胺类、多胺或α-甲基鸟氨酸的培养基中，并检测它们的rRNA合成。所有铵盐和胺类（但多胺除外）在1.25 - 5.0 mM浓度下都是rRNA合成的强效选择性抑制剂。α-甲基鸟氨酸虽然抑制鸟氨酸脱羧酶（一种据称是rRNA合成直接刺激因子的酶），但不抑制rRNA合成。在处理过程中，铵离子和单甲胺在处理过的细胞内积累。然而，单甲胺不会诱导铵离子的积累，反之亦然。铵盐和胺类也选择性抑制北极爪蟾神经胚细胞中的rRNA合成。
