Chen Jie, Mei Ming-Shun, Yu Yue, Zhao Yonglin, Gong Huan, Chen Weihao, Qiu Baoyu, Shi Songshan, Dilixiati Munisa, Wang Shunchun, Wang Huijun
The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
School of Chemical Science and Engineering, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China.
Int J Biol Macromol. 2024 Dec;283(Pt 2):137793. doi: 10.1016/j.ijbiomac.2024.137793. Epub 2024 Nov 16.
Oral administration of homogalacturonan (HG) has shown significant potential in anti-colitis activity, yet the therapeutic efficacy of naturally sourced HG still requires enhancement. Herein, HG from the fruits of Ficus pumila L. was modified by chemical methods and the intervention effect of modified HG with different degrees of methyl-esterification (DM) and acetylation (DA) on dextran sulfate sodium-induced colitis in mice was explored. Our results indicated that low-DM HG (DM3 and DM25) primarily mitigated colitis by reducing inflammation (TNF-α, IL-1β, IL-17, and IL-6), while high-DM HG (DM54 and DM94) primarily repaired the intestinal barrier. These effects may be attributed to the differential regulation of gut microbiota by HG with varying DM, such as Lachnospiraceae_NK4A136_group, Lactobacillus, Mucispirillum, Escherichia-Shigella, Bifidobacterium, and Bacteroides. Increased DA reduced the solubility of HG, showing limited anti-inflammatory response but unique advantages in intestinal barrier repair and microbiome regulation (Bifidobacterium, Candidatus_Saccharimonas, Lachnospiraceae_NK4A136_group, Mucispirillum, and Escherichia-Shigella). Furthermore, various structural parameters and substitution degrees showed no significant impact on HG's regulation of oxidative stress reactions. This study emphasized the importance of substituent effect in determining HG's functional role, providing a robust foundation for the design and development of functional polysaccharides for the prevention of intestinal inflammation and other related conditions.
口服高聚半乳糖醛酸（HG）已显示出显著的抗结肠炎活性潜力，但天然来源的HG的治疗效果仍需提高。在此，对薜荔果实中的HG进行化学修饰，并探讨不同甲酯化度（DM）和乙酰化度（DA）的修饰HG对葡聚糖硫酸钠诱导的小鼠结肠炎的干预作用。我们的结果表明，低DM的HG（DM3和DM25）主要通过减轻炎症（TNF-α、IL-1β、IL-17和IL-6）来缓解结肠炎，而高DM的HG（DM54和DM94）主要修复肠道屏障。这些作用可能归因于不同DM的HG对肠道微生物群的差异调节，如毛螺菌科_NK4A136_组、乳酸杆菌属、黏液螺旋菌属、埃希氏菌属-志贺氏菌属、双歧杆菌属和拟杆菌属。增加DA会降低HG的溶解度，显示出有限的抗炎反应，但在肠道屏障修复和微生物群调节（双歧杆菌属、候选糖单胞菌属、毛螺菌科_NK4A136_组、黏液螺旋菌属和埃希氏菌属-志贺氏菌属）方面具有独特优势。此外，各种结构参数和取代度对HG调节氧化应激反应没有显著影响。本研究强调了取代基效应在确定HG功能作用中的重要性，为预防肠道炎症和其他相关病症的功能性多糖的设计和开发提供了坚实基础。
