Zhang Jixiang, Sun Zhenou, Cheng Liting, Kang Ji, Liu Yan, Zhao Yi, Xiao Meng, Liu Huanhuan, Zhu Qiaomei, Guo Qingbin, Lin Changbin
State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
College of Food and Health, Zhejiang Agriculture and Forestry University, No. 666 Wusu Road, Linan District, Hangzhou 311300 Zhejiang, China.
J Agric Food Chem. 2025 Jan 15;73(2):1630-1641. doi: 10.1021/acs.jafc.4c07611. Epub 2025 Jan 1.
Polysaccharides from L. were investigated for their structural characterization and anti-inflammatory activity. Four low polymer dispersity index (PDI) subfractions were obtained: DRP-1 (153.95 kDa), DRP-2 (61.22 kDa), DRP-3 (22.80 kDa), and DRP-4 (8.93 kDa), respectively. DRP-4 contained the highest number of RG-I domains (43.25%), while DRP-1 had the highest degree of methyl esterification (37.5%). Results from the dextran sulfate sodium (DSS) salt-induced ulcerative colitis (UC) mice model indicated that DCP promoted mucosal and tight junction protein (caudin-1 and occludin) expression. Moreover, DCP improved the microbial community composition through selective enrichment of beneficial bacteria such as and . The anti-inflammatory activity of DCP was speculated to be related to its neutral sugar side chain and low esterification degree. These results suggested that DCP could prevent DSS-induced colitis and inhibit colon inflammation by maintaining a balanced gut microbiome and protecting the colon mucosal barrier.
对来自L.的多糖进行了结构表征和抗炎活性研究。获得了四个低聚合物分散指数（PDI）亚组分：分别为DRP-1（153.95 kDa）、DRP-2（61.22 kDa）、DRP-3（22.80 kDa）和DRP-4（8.93 kDa）。DRP-4含有最高数量的RG-I结构域（43.25%），而DRP-1具有最高的甲酯化程度（37.5%）。葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）小鼠模型的结果表明，DCP促进了黏膜和紧密连接蛋白（闭合蛋白-1和闭合蛋白）的表达。此外，DCP通过选择性富集有益细菌如 和 改善了微生物群落组成。推测DCP的抗炎活性与其中性糖侧链和低酯化程度有关。这些结果表明，DCP可以通过维持肠道微生物群平衡和保护结肠黏膜屏障来预防DSS诱导的结肠炎并抑制结肠炎症。
