Ao Yu-Tian, Dong Kai-Qiang, Wang Wen-Bo, Li Hong-Yao, Li Jia-Xiu, Guo Rong-Juan
Beijing University of Chinese Medicine, Beijing 100029, China.
Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078.
Zhen Ci Yan Jiu. 2024 Nov 25;49(11):1181-1189. doi: 10.13702/j.1000-0607.20240331.
To explore the effect of "Xingshen-Jieyu" (inducing resuscitation and dispel ling melancholy) electroacupuncture (EA) on serum metabolites in patients with depression and its underlying mechanisms through serum non-targeted metabolomics.
Fifteen depression patients were subjected into the EA group, and 15 healthy volunteers were matched into the healthy group. EA (2 Hz/15 Hz, a tolerable strength) was applied to Baihui (GV20), Shenting (GV24), Toulinqi (GB15), Benshen (GB13), Touwei (ST8), Xuanli (GB6) and Qubin (GB7) for 30 min, 3 times a week for 6 weeks. The severity of depression was assessed using Hamilton Depression Scale-17 (HAMD-17) and Hamilton Anxiety Scale (HAMA), the degree of fatigue was evaluated using Fatigue Scale-14 (FS-14). The subjects sleep quality was assessed using Pittsburgh sleep quality index (PSQI). Blood samples were collected before and after the treatment for processing serum to perform non-targeted metabolomics profile detection using ultra-high-performance liquid chromatography coupled with mass spectrometry (UPLC-MS), and Partial Least Squares Discriminant Analysis (PLS-DA, a statistical approach). Differential metabolites were screened and pathway enrichment analysis was performed.
After the treatment, the scores of HAMD-17, HAMA, PSQI and FS-14 of depression patients were significantly lowered in the EA group (<0.05). Compared with the healthy subjects, 46 differential metabolites (21 up-regulated, 25 down-regulated) were screened in patients with depression. Compared with pre-treatment in the EA group, 19 differential (17 up-regulated, and 2 down-regulated) metabolites were screened. Four down-regulated metabolites of the 46 differential metabolites in depression patients were recovered after EA treatment. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differential metabolites before and after EA showed that 19 metabolic pathways were enriched, mainly involving glutaminergic synapses, GABAergic synapses and several amino acid metabolism related pathways.
"Xingshen-Jieyu" EA mainly regulates the metabolism of amino acids, and activities of neurotransmitter glutamate and GABA, and restores the excitatory/inhibitory balance, thus playing a role in improving depression.
通过血清非靶向代谢组学探讨“醒神解郁”电针（EA）对抑郁症患者血清代谢产物的影响及其潜在机制。
将15例抑郁症患者纳入EA组，15名健康志愿者匹配为健康组。采用2Hz/15Hz、可耐受强度的电针刺激百会（GV20）、神庭（GV24）、头临泣（GB15）、本神（GB13）、头维（ST8）、悬厘（GB6）和曲鬓（GB7），每次30分钟，每周3次，共6周。采用汉密尔顿抑郁量表17项（HAMD-17）和汉密尔顿焦虑量表（HAMA）评估抑郁严重程度，采用疲劳量表14项（FS-14）评估疲劳程度。采用匹兹堡睡眠质量指数（PSQI）评估受试者的睡眠质量。治疗前后采集血样处理血清，采用超高效液相色谱-质谱联用（UPLC-MS）和偏最小二乘判别分析（PLS-DA，一种统计方法）进行非靶向代谢组学图谱检测。筛选差异代谢产物并进行通路富集分析。
治疗后，EA组抑郁症患者的HAMD-17、HAMA、PSQI和FS-14评分显著降低（<0.05）。与健康受试者相比，抑郁症患者筛选出46种差异代谢产物（21种上调，25种下调）。与EA组治疗前相比，筛选出19种差异代谢产物（17种上调，2种下调）。抑郁症患者46种差异代谢产物中有4种下调代谢产物经EA治疗后恢复。对EA前后差异代谢产物进行京都基因与基因组百科全书（KEGG）分析，发现19条代谢通路富集，主要涉及谷氨酸能突触、γ-氨基丁酸能突触和几条氨基酸代谢相关通路。
“醒神解郁”电针主要调节氨基酸代谢、神经递质谷氨酸和γ-氨基丁酸的活性，恢复兴奋/抑制平衡，从而发挥改善抑郁的作用。
