Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas - UNICAMP, Av. Alexandre Cazellato, 999, Paulínia, SP, 13148-218, Brazil.
Graduate Program in Genetics and Molecular Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255, Cidade Universitária, Campinas, SP, 13083-862, Brazil.
Biodegradation. 2024 Nov 18;36(1):7. doi: 10.1007/s10532-024-10100-6.
Spills of petroleum or its derivatives in the environment lead to an enrichment of microorganisms able to degrade such compounds. The interactions taking place in such microbial communities are complex and poorly understood, since they depend on multiple factors, including diversity and metabolic potential of the microorganisms and a broad range of fluctuating environmental conditions. In our previous study, a complete characterization, based on high-throughput sequencing, was performed in a jet-fuel plume using soil samples and in in-situ microcosms amended with hydrocarbons and exposed for 120 days. Herein, we propose a metabolic model to describe the monoaromatic hydrocarbon degradation process that takes place in such jet-fuel-contaminated sites, by combining genome-centered analysis, functional predictions, and flux balance analysis (FBA). In total, twenty high/medium quality MAGs were recovered; three of them assigned to anaerobic bacteria (Thermincolales, Geobacter and Pelotomaculaceace) and one affiliated to the aerobic bacterium Acinetobacter radioresistens, potentially the main players of hydrocarbon degradation in jet-fuel plumes. Taxonomic assignment of the genes indicated that a putative new species of Geobacteria has the potential for anaerobic degradation pathway, while the Pelotomaculaceae and Thermincolales members probably act via syntrophy oxidizing acetate and hydrogen (fermentation products of oil degradation) via sulfate and/or nitrate reduction.
环境中石油或其衍生物的泄漏会导致能够降解此类化合物的微生物的富集。这些微生物群落中的相互作用非常复杂，目前还不太了解，因为它们取决于多种因素，包括微生物的多样性和代谢潜力以及广泛变化的环境条件。在我们之前的研究中，使用土壤样本和添加了碳氢化合物并暴露了 120 天的原位微宇宙，基于高通量测序对喷气燃料羽流进行了完整的表征。在这里，我们通过组合基于基因组的分析、功能预测和通量平衡分析（FBA），提出了一个代谢模型来描述发生在这种喷气燃料污染场所的单芳烃烃降解过程。总共回收了二十个高质量/中等质量的 MAG；其中三个被分配给厌氧菌（Thermincolales、Geobacter 和 Pelotomaculaceace），一个与需氧菌 Acinetobacter radioresistens 相关，后者可能是喷气燃料羽流中烃类降解的主要参与者。基因的分类学分配表明，一种假定的新型 Geobacteria 具有厌氧降解途径的潜力，而 Pelotomaculaceae 和 Thermincolales 成员可能通过硫酸盐和/或硝酸盐还原来通过乙酸和氢气（石油降解的发酵产物）进行共生氧化。
