The Lotus corniculatus MYB5 functions as a master regulator in proanthocyanidin biosynthesis and bioengineering.

PAs varied greatly in leaves of different germplasm accessions in Lotus corniculatus and over-expression of LcMYB5 led to high PA accumulation in L. japonicus hairy roots. Proanthocyanidins (PAs) content in leaves is an important quality trait in forage species. The leaves of most forage crops accumulated no or little PAs, which makes it difficult to discover key genes involved in PA biosynthesis in the leaves. We found PAs content varied greatly in leaves of different germplasm accessions in Lotus corniculatus, which is one of the most agriculturally important forage crops. Through a combination of global transcriptional analysis, GO and KEGG analysis, and phylogenetic analysis, we discovered that LcMYB5 was strongly correlated with PA accumulation in leaves of L. corniculatus. The subcellular localization and transactivation activity assays demonstrated that LcMYB5 localized to the nucleus and acted as a transcriptional activator. Over-expression of the two homologs of LcMYB5 (LcMYB5a and LcMYB5b) in the L. japonicus hairy roots resulted in a particular high level of PAs. Global transcriptional analysis and qRT-PCR assays indicated that LcMYB5a and LcMYB5b up-regulated the transcript levels of many key PA pathway genes in the transgenic hairy roots, including structural genes (eg. CHS, F3H, LAR, ANR, and TT15) and regulatory genes (eg. TT8 and TTG1). Collectively, our data suggests that LcMYB5 independently regulates PA accumulation in the leaves of Lotus as a master regulator, which can be bioengineered for PAs production in the leaves of forage species.