LINC00852/miR-145-5p在乳腺癌中的重要性：一项生物信息学与实验研究

Importance of LINC00852/miR-145-5p in breast cancer: a bioinformatics and experimental study.

作者信息

Shakoori Abbas, Hosseinzadeh Asghar, Nafisi Nahid, Omranipour Ramesh, Sahebi Leyla, Ahmadi Mohsen, Ghafouri-Fard Soudeh, Abtin Maryam

机构信息

Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Department of Medical Genetics, Cancer Institute of Iran, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Dr. Qarib St., Keshavarz Blvd, Tehran, Iran.

出版信息

Discov Oncol. 2024 Nov 18;15(1):672. doi: 10.1007/s12672-024-01553-5.

PURPOSE

We aimed to examine the importance of an lncRNA, namely LINC00852, in the pathogenesis of breast cancer.

MATERIALS AND METHODS

In the current study, we used several online tools to examine the importance of LINC00852 in breast cancer. Then, we examined these findings in 50 pairs of breast cancer tissues and adjacent non-cancerous ones. We also re-evaluated the data of miR-145-5p signature from our recent study.

RESULTS

While in silico tools revealed down-regulation of LINC00852 in breast cancer samples, expression assays showed significant up-regulation of this lncRNAs in breast cancer samples compared with matching control samples from Iranian patients. miR-145-5p was under-expressed in breast cancer samples compared with non-cancerous samples. LINC00852 could separate breast cancer tissues from adjacent non-malignant tissues with an AUC value of 0.7218 (P value < 0.001).

CONCLUSION

The current study potentiates LINC00852/miR-145-5p axis as a possible contributor to the pathogenesis of breast cancer.

目的

我们旨在研究一种长链非编码RNA（lncRNA），即LINC00852在乳腺癌发病机制中的重要性。

材料与方法

在本研究中，我们使用了几种在线工具来研究LINC00852在乳腺癌中的重要性。然后，我们在50对乳腺癌组织和相邻的非癌组织中验证了这些发现。我们还重新评估了我们最近研究中的miR-145-5p特征数据。

结果

虽然计算机模拟工具显示乳腺癌样本中LINC00852表达下调，但表达分析显示，与来自伊朗患者的匹配对照样本相比，该lncRNA在乳腺癌样本中显著上调。与非癌样本相比，miR-145-5p在乳腺癌样本中表达不足。LINC00852能够将乳腺癌组织与相邻的非恶性组织区分开来，曲线下面积（AUC）值为0.7218（P值<0.001）。