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LINC00852通过miR-625/E2F1轴调控肝细胞癌的细胞增殖、侵袭、迁移和凋亡。

LINC00852 Regulates Cell Proliferation, Invasion, Migration and Apoptosis in Hepatocellular Carcinoma the miR-625/E2F1 Axis.

作者信息

Chen Shi

机构信息

Department of Pathology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, No. 283, Tongzipo Road, Yuelu District, Changsha, 410013 Hunan People's Republic of China.

出版信息

Cell Mol Bioeng. 2021 Dec 3;15(2):207-217. doi: 10.1007/s12195-021-00714-8. eCollection 2022 Apr.

DOI:10.1007/s12195-021-00714-8
PMID:35401844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8938543/
Abstract

OBJECTIVE: This study investigates the function and regulatory mechanism of lncRNA LINC00852 in hepatocellular carcinoma (HCC) cells. METHODS: The effects of LINC00852 and E2F1 on HCC cell proliferation, invasion, migration and apoptosis were measured by MTT assay, Transwell invasion and migration assays and TUNEL staining, respectively. The apoptosis of HCC cells was further determined by the expression levels of apoptosis-related proteins (Bax and Bcl2). Dual-luciferase reporter assays verified the targeting relationships among LINC00852, miR-625 and E2F1. RESULTS: Overexpression of LINC00852 was positively associated with HCC cell proliferation, invasion and migration while negatively associated with the cell apoptosis. LINC00852 bound miR-625 which further targeted E2F1. Overexpressing miR-625 or down-regulating E2F1 reversed the oncogenic effects of LINC00852. CONCLUSION: LINC00852 regulates HCC cell activities the miR-625/E2F1 axis.

摘要

目的：本研究探讨长链非编码RNA LINC00852在肝癌（HCC）细胞中的功能及调控机制。 方法：分别采用MTT法、Transwell侵袭和迁移实验以及TUNEL染色检测LINC00852和E2F1对肝癌细胞增殖、侵袭、迁移及凋亡的影响。通过凋亡相关蛋白（Bax和Bcl2）的表达水平进一步确定肝癌细胞的凋亡情况。双荧光素酶报告基因实验验证了LINC00852、miR-625和E2F1之间的靶向关系。 结果：LINC00852的过表达与肝癌细胞的增殖、侵袭和迁移呈正相关，而与细胞凋亡呈负相关。LINC00852与miR-625结合，miR-625进一步靶向E2F1。过表达miR-625或下调E2F1可逆转LINC00852的致癌作用。 结论：LINC00852通过miR-625/E2F1轴调节肝癌细胞活性。

相似文献

[1]
LINC00852 Regulates Cell Proliferation, Invasion, Migration and Apoptosis in Hepatocellular Carcinoma the miR-625/E2F1 Axis.

Cell Mol Bioeng. 2021-12-3

[2]
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[3]
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[4]
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[5]
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[6]
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[7]
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Ann Hepatol. 2021

[8]
LINC00852 is associated with poor prognosis in non-small cell lung cancer patients and its inhibition suppresses cancer cell proliferation and chemoresistance via the hsa-miR-145-5p/KLF4 axis.

J Gene Med. 2021-12

[9]
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Ann Hepatol. 2021

[10]
Histone demethylase KDM5D represses the proliferation, migration and invasion of hepatocellular carcinoma through the E2F1/TNNC1 axis.

Antioxid Redox Signal. 2024-3-19

引用本文的文献

[1]
L. Extract Inhibits Colorectal Cancer Tumor Growth by Modulating Cell Apoptosis and Autophagy.

Curr Issues Mol Biol. 2025-2-17

[2]
Importance of LINC00852/miR-145-5p in breast cancer: a bioinformatics and experimental study.

Discov Oncol. 2024-11-18

[3]
Current concepts of the crosstalk between lncRNA and E2F1: shedding light on the cancer therapy.

Front Pharmacol. 2024-7-25

[4]
Serum microRNA Profiles and Pathways in Hepatitis B-Associated Hepatocellular Carcinoma: A South African Study.

Int J Mol Sci. 2024-1-12

[5]
Differentially expressed non-coding RNAs and their regulatory networks in liver cancer.

Heliyon. 2023-8-19

[6]
CHCHD2 and CHCHD10: Future therapeutic targets in cognitive disorder and motor neuron disorder.

Front Neurosci. 2022-8-18

本文引用的文献

[1]
Exosome-transmitted linc00852 associated with receptor tyrosine kinase AXL dysregulates the proliferation and invasion of osteosarcoma.

Cancer Med. 2020-9

[2]
A potential prognostic prediction model of colon adenocarcinoma with recurrence based on prognostic lncRNA signatures.

Hum Genomics. 2020-6-10

[3]
Identification of Potential Prognostic Long Non-Coding RNA Biomarkers for Predicting Recurrence in Patients with Cervical Cancer.

Cancer Manag Res. 2020-1-31

[4]
LINC00958 facilitates cervical cancer cell proliferation and metastasis by sponging miR-625-5p to upregulate LRRC8E expression.

J Cell Biochem. 2020-3

[5]
miR-625 reverses multidrug resistance in gastric cancer cells by directly targeting ALDH1A1.

Cancer Manag Res. 2019-7-15

[6]
lncRNA-PDPK2P promotes hepatocellular carcinoma progression through the PDK1/AKT/Caspase 3 pathway.

Mol Oncol. 2019-8-22

[7]
E2F1 Drives Breast Cancer Metastasis by Regulating the Target Gene FGF13 and Altering Cell Migration.

Sci Rep. 2019-7-24

[8]
Olive Leaf Extract Attenuates Inflammatory Activation and DNA Damage in Human Arterial Endothelial Cells.

Front Cardiovasc Med. 2019-5-16

[9]
A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis.

Mol Cancer. 2019-2-19

[10]
LINC00852 Promotes Lung Adenocarcinoma Spinal Metastasis by Targeting S100A9.

J Cancer. 2018-10-18

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