Department of Botany, Government College Women University, Sialkot, Pakistan.
Sci Rep. 2024 Nov 18;14(1):28514. doi: 10.1038/s41598-024-78725-4.
In this study, sufaid chonsa mango pulp aqueous extracts from different ripening stages (RS I-V) was utilized to synthesize silver nanoparticles (Ag NPs). The Ag NPs were characterized using UV-vis spectrometry, X-ray diffraction analysis (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy dispersice X-ray analysis (EDX). Additionally, antioxidative potential and phenolic and flavonoid-like properties of synthesized Ag NPs were also accessed. UV-vis spectrophotometer analysis showed peaks in around 400 nm. XRD analysis confirmed the crystalline structure of the green-synthesized Ag NPs, with sizes ranging from 2.11 to 11.72 nm. FTIR verified the attachment of functional groups from the mango pulp extract to the Ag NPs. SEM analyses revealed that the morphology of the Ag NPs was primarily spherical that were agglomerated. The total antioxidant capacity, measured by the DPPH assay, showed 51% radical scavenging activity for RSIII extract synthesized NPs. The highest total antioxidant capacity was observed to be 80.22 and 79.14 µg AAE/mg NPs by RSI and RSIV synthesized NPs, respectively, while the maximum total reduction potential was 28.67 µg AAE/mg for Ag NPs synthesized by RSII extract. Ag NPs derived from RSIV exhibited phenolic-like property of 70.84 µg GAE/mg, while those derived from RSII had a maximum flavonoid-like property of 35.37 µg QE/mg. This study demonstrates that mango pulp at different ripening stages produces Ag NPs with distinct characteristics, making them suitable for various environmental and biomedical applications.
在这项研究中，利用不同成熟阶段（RS I-V）的苏法德·昌萨芒果浆水提物合成了银纳米粒子（Ag NPs）。利用紫外-可见分光光度计、X 射线衍射分析（XRD）、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和能谱分析（EDX）对 Ag NPs 进行了表征。此外，还评估了合成 Ag NPs 的抗氧化潜力以及酚类和类黄酮特性。紫外-可见分光光度计分析显示，在约 400nm 处有峰。XRD 分析证实了绿色合成的 Ag NPs 的结晶结构，粒径范围为 2.11 至 11.72nm。FTIR 验证了芒果浆提取物中的功能基团与 Ag NPs 的结合。SEM 分析表明，Ag NPs 的形态主要为球形，存在团聚现象。通过 DPPH 测定法测量的总抗氧化能力显示，RSIII 提取物合成的 NPs 对自由基的清除活性为 51%。观察到的最高总抗氧化能力分别为 RSI 和 RSIV 合成的 NPs 中的 80.22 和 79.14µg AAE/mg NPs，而 RSII 提取物合成的 Ag NPs 的最大总还原电位为 28.67µg AAE/mg。RSIV 衍生的 Ag NPs 表现出 70.84µg GAE/mg 的类酚特性，而 RSII 衍生的 Ag NPs 具有最大的 35.37µg QE/mg 的类黄酮特性。本研究表明，不同成熟阶段的芒果浆可产生具有独特特性的 Ag NPs，使其适用于各种环境和生物医学应用。
