U Din Mehwish Mohy, Batool Andleeb, Ashraf Raja Shahid, Yaqub Atif, Rashid Aneeba, U Din Nazish Mohy
Department of Zoology, Dr. Nazir Ahmad Institute of Biological Sciences, Government College University, Lahore, 54000 Lahore, Pakistan.
Department of Chemistry, Institute of Chemical Sciences, Government College University, Lahore, 54000 Lahore, Pakistan.
ACS Omega. 2024 Apr 15;9(17):18909-18921. doi: 10.1021/acsomega.3c08927. eCollection 2024 Apr 30.
The paper presents the antibacterial and antioxidant activities of silver nanoparticles (AgNPs) when conjugated with two antibiotics levofloxacin and ciprofloxacin as well as biologically synthesized nanoparticles from and . Leaves of and powder of were used in the green synthesis of silver nanoparticles. Ultraviolet-visible spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) were used for the characterization of the synthesized silver nanoparticles. Comparison of levofloxacin and ciprofloxacin and their conjugated AgNPs was also studied for antibacterial and antioxidant activity. The synthesis of -AgNPs, turmeric-AgNPs, levofloxacin-AgNPs, and ciprofloxacin-AgNPs was confirmed by UV spectroscopy. An absorption peak value of 400-450 nm was observed, and light to dark brown color indicated the synthesis of AgNPs. -AgNPs revealed high antioxidant activity (80.3 ± 3.14) among all of the synthesized AgNPs. Lev-AgNPs displayed the highest zone of inhibition for , while in , Cip-AgNPs showed high antibacterial activity. Furthermore, AgNPs synthesized using green methods exhibit high and efficient antimicrobial activities against two food-borne pathogens. Biologically synthesized nanoparticles exhibited antibacterial activity against (13.73 ± 0.46 with Tur-AgNPs and 13.53 ± 0.32 with Mor-AgNPs) and (14.16 ± 0.24 with Tur-AgNPs and 13.36 ± 0.77 with Mor-AgNPs) by using a well diffusion method with significant shrinkage and damage of the bacterial cell wall, whereas antibiotic-conjugated nanoparticles showed high antibacterial activity compared to biologically synthesized nanoparticles with 14.4 ± 0.37 for Cip-AgNPs and 13.93 ± 0.2 for Lev-AgNPs for and 13.3 ± 0.43 for Cip-AgNPs and 14.33 ± 0.12 for Lev-AgNPs for . The enhanced efficiency of conjugated silver nanoparticles is attributed to their increased surface area compared to larger particles. Conjugation of different functional groups contributes to improved reactivity, creating active sites for catalytic reactions. Additionally, the precise control over the size and shape of green-synthesized nanoparticles further augments their catalytic and antibiotic activities.
本文介绍了银纳米颗粒（AgNPs）与两种抗生素左氧氟沙星和环丙沙星结合时的抗菌和抗氧化活性，以及由[植物名称1]和[植物名称2]生物合成的纳米颗粒的抗菌和抗氧化活性。[植物名称1]的叶子和[植物名称2]的粉末用于银纳米颗粒的绿色合成。采用紫外可见光谱（UV）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）对合成的银纳米颗粒进行表征。还研究了左氧氟沙星和环丙沙星及其共轭AgNPs的抗菌和抗氧化活性比较。通过紫外光谱证实了[植物名称1]-AgNPs、姜黄-AgNPs、左氧氟沙星-AgNPs和环丙沙星-AgNPs的合成。观察到吸收峰值在400 - 450 nm之间，颜色从浅到深褐色表明AgNPs的合成。在所有合成的AgNPs中，[植物名称1]-AgNPs显示出高抗氧化活性（80.3±3.14）。左氧氟沙星-AgNPs对[细菌名称1]显示出最大抑菌圈，而在[细菌名称2]方面，环丙沙星-AgNPs表现出高抗菌活性。此外，采用绿色方法合成的AgNPs对两种食源性病原体具有高效抗菌活性。通过平板扩散法，生物合成的纳米颗粒对[细菌名称1]（姜黄-AgNPs为13.73±0.46，[植物名称1]-AgNPs为13.53±0.32）和[细菌名称2]（姜黄-AgNPs为14.16±0.24，[植物名称1]-AgNPs为13.36±0.77）表现出抗菌活性，细菌细胞壁有明显收缩和损伤，而抗生素共轭纳米颗粒相比生物合成的纳米颗粒表现出更高的抗菌活性（环丙沙星-AgNPs对[细菌名称1]为14.4±0.37，左氧氟沙星-AgNPs为13.93±0.2；环丙沙星-AgNPs对[细菌名称2]为13.3±0.43，左氧氟沙星-AgNPs为14.33±0.12）。共轭银纳米颗粒效率的提高归因于与较大颗粒相比其表面积增加。不同官能团的共轭有助于提高反应活性，为催化反应创造活性位点。此外，对绿色合成纳米颗粒尺寸和形状的精确控制进一步增强了它们的催化和抗生素活性。
