Rifai N, King M E, Malekpour A, Smith J, Lawson J
Clin Biochem. 1986 Feb;19(1):31-4. doi: 10.1016/s0009-9120(86)80068-6.
The introduction of specific antisera has led to the replacement of total iron binding capacity assays with direct immunochemical measures of transferrin. However, some controversy exists over the effect of the degree of iron saturation on antigen-antibody interactions in these immunochemical assays. This study describes a simple automated immunoturbidimetric assay of transferrin, and includes a reference range and an evaluation of the effects of common potential interferents, including iron, bilirubin, lipemia, and hemoglobin. The measured and the calculated TIBC are also compared for 78 healthy individuals and 51 patients with anemia.
特异性抗血清的引入使得用转铁蛋白的直接免疫化学检测方法取代总铁结合力检测成为可能。然而，在这些免疫化学检测中，铁饱和度程度对抗抗原-抗体相互作用的影响仍存在一些争议。本研究描述了一种简单的转铁蛋白自动免疫比浊法，包括参考范围以及对常见潜在干扰物（包括铁、胆红素、脂血和血红蛋白）影响的评估。还对78名健康个体和51名贫血患者的实测和计算总铁结合力进行了比较。
