Kreutzer H J
J Clin Chem Clin Biochem. 1976 Aug;14(8):401-6. doi: 10.1515/cclm.1976.14.1-12.401.
The described method for the turbidimetric determination of serum transferrin concentration has the following features: the ionic strength of the medium is very low, the antiserum used is purified by the manufacturer, 25 mul antiserum per determination is sufficient, excess antibody or antigen has no influence. We studied the relation between transferrin concentration and total iron binding capacity for human normal and variant transferrins. Furthermore, our results are compared with the radial immunodiffusion technique.
介质的离子强度非常低,所用抗血清由制造商纯化,每次测定25微升抗血清就足够,过量的抗体或抗原没有影响。我们研究了人正常和变异转铁蛋白的转铁蛋白浓度与总铁结合能力之间的关系。此外,我们将结果与放射免疫扩散技术进行了比较。