Vaidya H C, Maynard Y, Dietzler D N, Ladenson J H
Clin Chem. 1986 Apr;32(4):657-63.
Fusion of splenocytes from A/J mice immunized by creatine kinase (EC 2.7.3.2)-MB isoenzyme (CK-MB) with SP2/0-Ag14 myeloma cell line generated hybridomas producing monoclonal antibodies specific to CK-MB. One of these monoclonal antibodies ("Conan-MB") was used to develop a direct assay for CK-MB activity. In the assay, serum is incubated for 30 min at room temperature with "Conan-MB" immobilized on latex beads. The beads are then washed, and CK-MB activity bound to the antibody is measured after incubation with CK enzyme reagent for 30 min at 37 degrees C. Results with the assay correlated well (r = 0.997) with those for CK-MB concentration as measured by a two-site immunoassay. Neither CK-MM, CK-BB, mitochondrial CK, nor a hemolysate of erythrocytes interfered. Use of this unique monoclonal antibody allows rapid, precise, and direct determination of CK-MB activity.
用肌酸激酶（EC 2.7.3.2）-MB同工酶（CK-MB）免疫的A/J小鼠脾细胞与SP2/0-Ag14骨髓瘤细胞系融合，产生了能分泌针对CK-MB的单克隆抗体的杂交瘤。其中一种单克隆抗体（“Conan-MB”）被用于开发一种直接检测CK-MB活性的方法。在该检测中，血清在室温下与固定在乳胶珠上的“Conan-MB”孵育30分钟。然后洗涤珠子，并在37℃下与CK酶试剂孵育30分钟后，测量与抗体结合的CK-MB活性。该检测结果与通过双位点免疫测定法测得的CK-MB浓度结果具有良好的相关性（r = 0.997）。CK-MM、CK-BB、线粒体CK以及红细胞溶血产物均无干扰。使用这种独特的单克隆抗体可实现对CK-MB活性的快速、精确和直接测定。
