栀子果提取物可缓解大鼠非甾体抗炎药相关性胃病。
Gardenia jasminoides fruit extract alleviates non-steroidal anti-inflammatory drug-induced gastropathy in rats.
机构信息
Master of Science Program in Medical Sciences, Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Center of Excellence in Alternative and Complementary Medicine for Gastrointestinal and Liver Diseases, Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
出版信息
BMC Complement Med Ther. 2024 Nov 18;24(1):401. doi: 10.1186/s12906-024-04702-z.
BACKGROUND
NSAID-induced gastropathy is a health burden that requires effective intervention. Among various prevention options, Gardenia jasminoides fruit extract (GJE) has demonstrated gastroprotective effects through anti-inflammatory pathways with a wide safety margin. However, the detailed molecular mechanisms of GJE regarding mucoprotective and anti-inflammatory effects remained to be explored. Therefore, we investigated the effects of GJE on NSAID-induced gastric injury in rats, focusing on the expression of the protective factors: prostaglandin E (PGE) and mucin 5AC (MUC5AC), and the aggravating factors: inducible nitric oxide synthase (iNOS) and nuclear factor-κB (NF-κB).
METHODS
Twenty-four male Sprague-Dawley rats were assigned to three experimental groups (n = 8/group): the control group, the NSAIDs group receiving indomethacin to induce gastric ulcers, and the NSAIDs with GJE pretreatment (NSAIDs + GJE) group. After a two-day experimental period, the stomachs were collected for histopathological examination, immunohistochemical staining, and protein expression analysis in gastric tissue lysates.
RESULTS
The NSAIDs group exhibited severe neutrophil infiltration with ulcers upon gastric histopathological examination. Pretreatment with GJE attenuated NSAID-induced gastropathy, as evidenced by reduced neutrophil infiltration and decreased ulceration. Immunohistochemical staining and Western blotting demonstrated reduced expressions of PGE and MUC5AC, while the expressions of iNOS and NF-κB were increased following NSAID administration. In comparison to the NSAIDs group, the NSAIDs + GJE group exhibited higher expressions of PGE and MUC5AC and lower expressions of iNOS and NF-κB, providing evidence of the gastroprotective effects of GJE.
CONCLUSIONS
Pretreatment with GJE alleviated NSAID-induced gastric ulcers by increasing the expression of PGE and MUC5AC and decreasing the expression of iNOS and NF-κB. This study contributes to the understanding of the mechanisms by which GJE attenuates NSAID-induced gastropathy. Further studies are required to validate the effect of GJE in clinical settings.
背景
非甾体抗炎药（NSAID）诱导的胃病是一种需要有效干预的健康负担。在各种预防选择中，栀子果提取物（GJE）通过抗炎途径显示出胃保护作用，且具有较宽的安全范围。然而，GJE 关于黏膜保护和抗炎作用的详细分子机制仍有待探索。因此，我们研究了 GJE 对大鼠 NSAID 诱导性胃损伤的影响，重点关注保护因子：前列腺素 E（PGE）和黏蛋白 5AC（MUC5AC）的表达，以及加重因子：诱导型一氧化氮合酶（iNOS）和核因子-κB（NF-κB）的表达。
方法
将 24 只雄性 Sprague-Dawley 大鼠随机分为三组（每组 8 只）：对照组、给予吲哚美辛诱导胃溃疡的 NSAIDs 组和 NSAIDs 加 GJE 预处理（NSAIDs+GJE）组。在为期两天的实验期间，收集胃进行组织学检查、免疫组织化学染色和胃组织裂解物中的蛋白表达分析。
结果
NSAIDs 组胃组织学检查显示严重的中性粒细胞浸润和溃疡。GJE 预处理减轻了 NSAID 诱导的胃病，表现为中性粒细胞浸润减少和溃疡减少。免疫组织化学染色和 Western blot 显示，PGE 和 MUC5AC 的表达减少，而 iNOS 和 NF-κB 的表达增加。与 NSAIDs 组相比，NSAIDs+GJE 组 PGE 和 MUC5AC 的表达增加，iNOS 和 NF-κB 的表达减少，表明 GJE 具有胃保护作用。
结论
GJE 预处理通过增加 PGE 和 MUC5AC 的表达和减少 iNOS 和 NF-κB 的表达，减轻 NSAID 诱导的胃溃疡。本研究有助于了解 GJE 减轻 NSAID 诱导性胃病的机制。需要进一步的研究来验证 GJE 在临床环境中的效果。
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