John Sharon, Khan Eram, Jain Ayushi, Devi Priya, Gupta Shalini
Department of Oral Pathology, King George's Medical University, Lucknow, UP 226003 India.
Department of Oral & Maxillofacial Pathology and Oral Microbiology, King George's Medical University, Lucknow, India.
Indian J Otolaryngol Head Neck Surg. 2024 Dec;76(6):5610-5617. doi: 10.1007/s12070-024-05044-5. Epub 2024 Sep 9.
Odontogenic Keratocysts (OKCs) are known for their aggressive behavior along with rapid expansion. Subepithelial hyalinization (SEH) is one of the causes of recurrence. The ability to predict this biological behavior histologically may help medical experts choose the best course of action. To investigate the aggressiveness of odontogenic keratocyst caused by SEH and its recurrence tendency in the north Indian population, this study will link differential staining methods with immunohistochemistry biomarkers that can be used in routine investigative procedures. Consequently, the evaluation and grading of SEH were established by measuring from the basement membrane to the extent of connective tissue. The levels were correlated to Ki67, Alcian blue, and O -safranine for validation. Forty OKCs were examined for the histological investigation of SEH using the immunohistochemical marker Ki67 and differential staining with O-safranine and Alcian Blue. The histological trait of separation of epithelium from the connective tissue interface due to SEH was noted. SEH-positive cases that were evaluated with Ki67, had increased proliferative activity. The differential staining techniques were validated with Ki67, cross-tabulations in SPSS, and kappa statistic value was given to analyze the results. Spearman's rank correlation was done between Ki67 vs Alcian blue and O-Safranine. A value of less than < 0.05 was considered statistically significant. In SEH-positive cases, a higher proliferative index was observed. Additionally, histological metrics were statistically significantly higher in SEH-positive cases. Consequently, SEH is a reliable histopathological indicator in OKC for predicting recurrence. The presence of SEH indicates that OKCs are more likely to recur.
牙源性角化囊肿（OKCs）以其侵袭性生长和快速扩张而闻名。上皮下玻璃样变（SEH）是其复发的原因之一。从组织学上预测这种生物学行为的能力可能有助于医学专家选择最佳治疗方案。为了研究印度北部人群中由SEH引起的牙源性角化囊肿的侵袭性及其复发倾向，本研究将差异染色方法与可用于常规检查程序的免疫组化生物标志物联系起来。因此，通过测量从基底膜到结缔组织的范围来建立SEH的评估和分级。将这些水平与Ki67、阿尔辛蓝和O-番红进行相关性验证。使用免疫组化标志物Ki67以及O-番红和阿尔辛蓝差异染色对40个OKCs进行SEH的组织学研究。记录了由于SEH导致上皮与结缔组织界面分离的组织学特征。用Ki67评估的SEH阳性病例具有增加的增殖活性。差异染色技术通过Ki67进行验证，在SPSS中进行交叉表分析，并给出kappa统计值以分析结果。对Ki67与阿尔辛蓝和O-番红进行Spearman等级相关性分析。P值小于<0.05被认为具有统计学意义。在SEH阳性病例中，观察到较高的增殖指数。此外，SEH阳性病例的组织学指标在统计学上显著更高。因此，SEH是OKC中预测复发的可靠组织病理学指标。SEH的存在表明OKCs更有可能复发。
