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采用电化学法和E-Screen法评估植物生长调节剂的联合雌激素效应。

Evaluating the combined estrogenic effects of plant growth regulators electrochemical and E-Screen methods.

作者信息

Wang Xijie, Zhao Zijia, Qi Shulan, Li Zan, Wang Zhong, Zhou Shi, Cui Jiwen, Li Jinlian, Wu Dongmei

机构信息

College of Pharmacy, Jiamusi University Jiamusi Heilongjiang 154007 P. R. China

Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University Jiamusi 154007 China.

出版信息

RSC Adv. 2024 Nov 18;14(49):36745-36753. doi: 10.1039/d4ra06838f. eCollection 2024 Nov 11.

DOI:10.1039/d4ra06838f
PMID:39559573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11571117/
Abstract

The study shows that plant growth regulators (PGRs) have estrogenic effects, which may disrupt the normal physiological functions of endogenous estrogen in organisms. This study used electrochemical methods to investigate the electrochemical behavior and estrogenic effects of PGRs gibberellic acid (GA), ethylene (ETH), and naphthalene acetic acid (NAA) on estrogen-free human breast cancer cells (MCF-7) cells when exposed individually or in combination. The results indicate that GA, ETH, and NAA, whether used alone or in combination, exhibit estrogenic effects on MCF-7 cells. The accuracy of the electrochemical method was validated against the E-Screen method, with consistent results between the two methods. Analysis of the combined estrogenic effects of PGRs detected by electrochemical and E-Screen methods revealed antagonistic effects for GA/ETH, synergistic effects for GA/NAA, additive effects for NAA/ETH, and synergistic effects for GA/ETH/NAA. The combined estrogenic effects of PGRs at environmental actual concentration ratios detected by the electrochemical method were consistent with the results of the E-Screen method. This study successfully established a simple, fast, sensitive, and low-cost electrochemical detection method for the combined estrogenic effects of PGRs, providing a new approach for detecting such effects.

摘要

研究表明，植物生长调节剂（PGRs）具有雌激素效应，这可能会扰乱生物体中内源性雌激素的正常生理功能。本研究采用电化学方法，研究了植物生长调节剂赤霉素（GA）、乙烯（ETH）和萘乙酸（NAA）单独或联合作用于无雌激素的人乳腺癌细胞（MCF-7）时的电化学行为和雌激素效应。结果表明，GA、ETH和NAA无论是单独使用还是联合使用，对MCF-7细胞均表现出雌激素效应。通过与E-Screen方法对比验证了电化学方法的准确性，两种方法结果一致。对电化学和E-Screen方法检测到的植物生长调节剂联合雌激素效应分析表明，GA/ETH表现为拮抗作用，GA/NAA表现为协同作用，NAA/ETH表现为相加作用，GA/ETH/NAA表现为协同作用。电化学方法检测到的植物生长调节剂在环境实际浓度比下的联合雌激素效应与E-Screen方法的结果一致。本研究成功建立了一种简单、快速、灵敏、低成本的检测植物生长调节剂联合雌激素效应的电化学检测方法，为检测此类效应提供了一种新途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d58/11571117/43d6d32cd3d1/d4ra06838f-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d58/11571117/760bda408750/d4ra06838f-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d58/11571117/43d6d32cd3d1/d4ra06838f-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d58/11571117/760bda408750/d4ra06838f-f1.jpg
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本文引用的文献

1
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Pestic Biochem Physiol. 2023 Nov;196:105640. doi: 10.1016/j.pestbp.2023.105640. Epub 2023 Oct 4.
2
The response of electrochemical method to estrogen effect and the tolerance to culture factors: Comparison with MTT and cell counting methods.电化学法对雌激素效应的响应及其对培养因素的耐受性：与 MTT 和细胞计数法的比较。
Anal Chim Acta. 2022 Nov 15;1233:340514. doi: 10.1016/j.aca.2022.340514. Epub 2022 Oct 13.
3
Electrochemical detection mechanism of estrogen effect induced by cadmium: The regulation of purine metabolism by the estrogen effect of cadmium.
镉诱导雌激素效应的电化学检测机制：镉的雌激素效应对嘌呤代谢的调节。
Chemosphere. 2023 Jan;311(Pt 2):136970. doi: 10.1016/j.chemosphere.2022.136970. Epub 2022 Oct 22.
4
Role of endocrine disruption in toxicity of 6-benzylaminopurine (6-BA) to early-life stages of Zebrafish.内分泌干扰物在 6-苄基氨基嘌呤（6-BA）对斑马鱼早期发育阶段毒性中的作用。
Ecotoxicol Environ Saf. 2022 Mar 1;232:113287. doi: 10.1016/j.ecoenv.2022.113287. Epub 2022 Feb 8.
5
The Reproductive Journey in the Genomic Era: From Preconception to Childhood.基因组时代的生殖之旅：从孕前到儿童期。
Genes (Basel). 2020 Dec 19;11(12):1521. doi: 10.3390/genes11121521.
6
Effects of prepubertal exposure to forchlorfenuron through prenatal and postnatal gavage administration in developing Sprague-Dawley rats.产前和产后经口灌胃给予氯吡脲对发育中的斯普拉格-道利大鼠青春期前暴露的影响。
Reprod Toxicol. 2020 Dec;98:157-164. doi: 10.1016/j.reprotox.2020.09.009. Epub 2020 Sep 28.
7
Development of a paper-based microanalysis device doped with multi-walled carbon nanotubes for in vitro evaluation of fluorene cytotoxicity.基于纸的微分析器件的研制，掺杂多壁碳纳米管，用于芴体外细胞毒性评估。
Bioelectrochemistry. 2020 Oct;135:107552. doi: 10.1016/j.bioelechem.2020.107552. Epub 2020 May 12.
8
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Toxicol Lett. 2020 Feb 1;319:242-249. doi: 10.1016/j.toxlet.2019.10.025. Epub 2019 Nov 13.
9
A label-free approach to detect cell viability/cytotoxicity based on intracellular xanthine/guanine by electrochemical method.
J Pharmacol Toxicol Methods. 2019 Nov-Dec;100:106625. doi: 10.1016/j.vascn.2019.106625. Epub 2019 Aug 22.
10
180 Day Repeated-Dose Toxicity Study on Forchlorfenuron in Sprague-Dawley Rats and Its Effects on the Production of Steroid Hormones.氟啶脲在 Sprague-Dawley 大鼠中的 180 天重复剂量毒性研究及其对甾体激素产生的影响。
J Agric Food Chem. 2019 Sep 11;67(36):10207-10213. doi: 10.1021/acs.jafc.9b03855. Epub 2019 Aug 28.