Guangdong-Hong Kong Joint Innovation Platform for the Safety of Bakery Products, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
Guangdong-Hong Kong Joint Innovation Platform for the Safety of Bakery Products, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Engineering Technology Research Center, Guangzhou College of Technology and Business, 510580 Guangzhou, Guangdong, China.
Carbohydr Polym. 2025 Jan 15;348(Pt A):122822. doi: 10.1016/j.carbpol.2024.122822. Epub 2024 Oct 8.
Treating chronic wounds poses significant challenges in clinical medicine due to bacterial infection, reactive oxygen species (ROS) accumulation, and excessive inflammation. This study aimed to address these issues by developing a wound dressing with antibacterial, antioxidant, and anti-inflammatory properties. Chitosan was functionally modified with acrolein to covalently bind to epigallocatechin gallate (EGCG), enabling a high EGCG load. Subsequently, polyvinyl alcohol (PVA) and EGCG-modified chitosan were crosslinked to prepare a new double-network hydrogel with added cysteine (CSAEC/P50). CSAEC/P50 demonstrated optimal mechanical properties (low swelling rate, high water retention, and optimal flexibility), low hemolysis, high coagulation properties, and antibacterial and antioxidant activities. Cell scratch tests indicated that CSAEC/P50 can promote NIH3T3 cell migration. Immunofluorescence results showed that CSAEC/P50 promoted the transformation of proinflammatory M macrophages to anti-inflammatory M macrophages. These findings suggest that CSAEC/P50 has significant potential for use in wound dressing applications.
治疗慢性创伤在临床医学上面临着细菌感染、活性氧（ROS）积累和过度炎症等问题。本研究旨在通过开发具有抗菌、抗氧化和抗炎特性的创伤敷料来解决这些问题。壳聚糖通过丙烯醛的功能化修饰与表没食子儿茶素没食子酸酯（EGCG）共价结合，实现了高 EGCG 负载。随后，聚乙烯醇（PVA）和 EGCG 修饰的壳聚糖被交联，制备了一种新的双网络水凝胶，加入半胱氨酸（CSAEC/P50）。CSAEC/P50 表现出最佳的机械性能（低溶胀率、高保水率和最佳柔韧性）、低溶血率、高凝血性能以及抗菌和抗氧化活性。细胞划痕试验表明，CSAEC/P50 可以促进 NIH3T3 细胞迁移。免疫荧光结果表明，CSAEC/P50 促进了促炎 M 巨噬细胞向抗炎 M 巨噬细胞的转化。这些发现表明，CSAEC/P50 在创伤敷料应用方面具有很大的潜力。
