Orthopedic Department, Peking University First Hospital, Beijing, 100034, China.
Orthopedic Department, Peking University Third Hospital, Beijing, 100191, China.
Sci Rep. 2024 Nov 19;14(1):28599. doi: 10.1038/s41598-024-77270-4.
Peri-implant infection is a serious complication in orthopedic surgery. This study aimed to reduce the incidence of peri-implant infection by developing a durable and safe antibacterial silver coating. We compared the antibacterial properties and process controllability of various coating techniques to identify the most effective method for silver coating. We refined substrate treatment techniques and coating thicknesses through antibacterial and scratch tests. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were used to analyze the coating's morphology and composition. Micron-sized magnetron sputtering silver coating samples underwent in vitro antibacterial testing, cytotoxicity testing, silver ion release testing, and osteogenic testing using membrane contact culture, CCK-8 assay, inductively coupled plasma (ICP) detection, and alkaline phosphatase (ALP) activity/osteogenic gene PCR. Magnetron sputtering demonstrated superior antibacterial properties, uniformity, and process controllability compared to alternative techniques. The optimal adhesion strength was achieved with a 0.5 μm coating thickness and a 400 mesh sandpaper pretreatment process, without compromising antibacterial efficacy. The coating showed near-perfect antiseptic results in antibacterial and anti-biofilm tests. Fibroblasts cultured in silver ion precipitation medium exhibited growth rates of 89% on day 30 and 88% on day 90, compared to 95% in the control group. The osteogenic test indicated that the magnetron sputtering silver coating promotes osteogenesis effectively. Our study demonstrated that micron-sized magnetron sputtering silver coating has potential for clinical use to prevent peri-implant infections in the future.
种植体周围感染是骨科手术中的一种严重并发症。本研究旨在通过开发耐用且安全的抗菌银涂层来降低种植体周围感染的发生率。我们比较了各种涂层技术的抗菌性能和工艺可控性，以确定银涂层的最有效方法。我们通过抗菌和划痕试验改进了基底处理技术和涂层厚度。扫描电子显微镜（SEM）和能谱（EDS）用于分析涂层的形态和组成。使用膜接触培养、CCK-8 测定、电感耦合等离子体（ICP）检测和碱性磷酸酶（ALP）活性/成骨基因 PCR 对微米级磁控溅射银涂层样品进行体外抗菌试验、细胞毒性试验、银离子释放试验和成骨试验。与其他技术相比，磁控溅射显示出优越的抗菌性能、均匀性和工艺可控性。采用 0.5 μm 涂层厚度和 400 目砂纸预处理工艺，可获得最佳的结合强度，同时不影响抗菌效果。涂层在抗菌和抗生物膜试验中表现出近乎完美的防腐效果。在银离子沉淀培养基中培养的成纤维细胞在第 30 天和第 90 天的生长率分别为 89%和 88%，而对照组为 95%。成骨试验表明，磁控溅射银涂层能有效促进成骨。我们的研究表明，微米级磁控溅射银涂层具有在未来预防种植体周围感染的临床应用潜力。
