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一种新的癌症/睾丸长非编码 RNA，即 OTP-AS1 RNA。

A new cancer/testis long noncoding RNA, the OTP-AS1 RNA.

机构信息

Vavilov Institute of General Genetics, Moscow, Russia.

The Biomedical Center, St. Petersburg, Russia.

出版信息

Sci Rep. 2024 Nov 19;14(1):28676. doi: 10.1038/s41598-024-80065-2.

DOI:10.1038/s41598-024-80065-2
PMID:39562620
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11576910/
Abstract

The orthopedia homeobox (OTP) gene encodes a homeodomain-containing transcription factor involved in brain development. OTP is mapped to human chromosome 5q14.1. Earlier we described transcription in the second intron of this gene in wide variety of tumors, but among normal tissues only in testis. In GeneBank these transcripts are represented by several 300-400 nucleotide long AI267901-like ESTs. We assumed that the AI267901-like ESTs belonged to the longer transcript(s). We used the Rapid Amplification of cDNA Ends (RACE) approach and other methods to find the full-length transcript. The transcript we found was a 2436 nucleotide polyadenylated sequence in antisense to OTP gene. The corresponding gene consisted of two exons separated by an intron of 2961 bp. The first exon was found to be 91 bp long and located in the third exon of OTP. The second exon was 2345 bp long and located in the second intron of OTP. We have shown the expression of this gene in many human tumors but as few as a single sample of normal testis. The transcript lacked significant ORFs suggesting that we discovered a new antisense cancer/testis (CT) sequence OTP-AS1 (OTP-antisense RNA 1), which belongs to the class of long noncoding RNAs (lncRNAs). According to our findings we assume that OTP-AS1 and OTP genes may be a CT-coding gene/CT-ncRNA pair, or sense-antisense gene pair involved in regulatory interactions.

摘要

该 orthopedia 同源盒（OTP）基因编码一个含有同源域的转录因子，参与脑发育。OTP 定位于人类染色体 5q14.1。我们之前描述了这个基因的第二个内含子在广泛的肿瘤中转录，但在正常组织中仅在睾丸中转录。在 GeneBank 中，这些转录物由几个 300-400 个核苷酸长的 AI267901 样 EST 代表。我们假设 AI267901 样 EST 属于更长的转录物。我们使用快速扩增 cDNA 末端（RACE）方法和其他方法来寻找全长转录物。我们发现的转录物是一个 2436 个核苷酸长的多聚腺苷酸化序列，与 OTP 基因呈反义。相应的基因由两个外显子组成，中间隔有一个 2961 个核苷酸的内含子。第一个外显子长 91 个核苷酸，位于 OTP 的第三个外显子中。第二个外显子长 2345 个核苷酸，位于 OTP 的第二个内含子中。我们已经证明了该基因在许多人类肿瘤中的表达，但在正常睾丸中只有一个样本表达。该转录物缺乏显著的 ORF，这表明我们发现了一个新的反义癌/睾丸（CT）序列 OTP-AS1（OTP-反义 RNA 1），属于长非编码 RNA（lncRNA）类。根据我们的发现，我们假设 OTP-AS1 和 OTP 基因可能是一个 CT 编码基因/CT-ncRNA 对，或参与调节相互作用的有意义-反义基因对。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/e1f88c6480bc/41598_2024_80065_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/f5f967f46bb4/41598_2024_80065_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/0851cdef1167/41598_2024_80065_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/db01459124cf/41598_2024_80065_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/b277b47a2f91/41598_2024_80065_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/befdc77192c4/41598_2024_80065_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/a2ec2b738fb1/41598_2024_80065_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/108d8ecc723f/41598_2024_80065_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/e1f88c6480bc/41598_2024_80065_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/f5f967f46bb4/41598_2024_80065_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/0851cdef1167/41598_2024_80065_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/db01459124cf/41598_2024_80065_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/b277b47a2f91/41598_2024_80065_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/befdc77192c4/41598_2024_80065_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/a2ec2b738fb1/41598_2024_80065_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/108d8ecc723f/41598_2024_80065_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad39/11576910/e1f88c6480bc/41598_2024_80065_Fig8_HTML.jpg

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本文引用的文献

1
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Cell Death Dis. 2022 Mar 31;13(3):287. doi: 10.1038/s41419-022-04747-3.
2
ReMap 2022: a database of Human, Mouse, Drosophila and Arabidopsis regulatory regions from an integrative analysis of DNA-binding sequencing experiments.ReMap 2022：一个整合了 DNA 结合测序实验分析的人类、小鼠、果蝇和拟南芥调控区域数据库。
Nucleic Acids Res. 2022 Jan 7;50(D1):D316-D325. doi: 10.1093/nar/gkab996.
3
Multimodal Long Noncoding RNA Interaction Networks: Control Panels for Cell Fate Specification.
多模态长非编码 RNA 相互作用网络：细胞命运特化的调控面板。
Genetics. 2019 Dec;213(4):1093-1110. doi: 10.1534/genetics.119.302661.
4
Gene expression across mammalian organ development.哺乳动物器官发育过程中的基因表达。
Nature. 2019 Jul;571(7766):505-509. doi: 10.1038/s41586-019-1338-5. Epub 2019 Jun 26.
5
Modulating the expression of long non-coding RNAs for functional studies.调控长非编码 RNA 的表达进行功能研究。
EMBO Rep. 2018 Dec;19(12). doi: 10.15252/embr.201846955. Epub 2018 Nov 21.
6
GTRD: a database on gene transcription regulation-2019 update.GTRD：一个关于基因转录调控的数据库-2019 年更新。
Nucleic Acids Res. 2019 Jan 8;47(D1):D100-D105. doi: 10.1093/nar/gky1128.
7
Genome-wide map of human and mouse transcription factor binding sites aggregated from ChIP-Seq data.整合自染色质免疫沉淀测序（ChIP-Seq）数据的人类和小鼠转录因子结合位点全基因组图谱。
BMC Res Notes. 2018 Oct 23;11(1):756. doi: 10.1186/s13104-018-3856-x.
8
Functional Classification and Experimental Dissection of Long Noncoding RNAs.长非编码 RNA 的功能分类与实验解析。
Cell. 2018 Jan 25;172(3):393-407. doi: 10.1016/j.cell.2018.01.011.
9
HOCOMOCO: towards a complete collection of transcription factor binding models for human and mouse via large-scale ChIP-Seq analysis.HOCOMOCO：通过大规模的 ChIP-Seq 分析，构建人类和小鼠转录因子结合模型的完整集合。
Nucleic Acids Res. 2018 Jan 4;46(D1):D252-D259. doi: 10.1093/nar/gkx1106.
10
FANTOM5 CAGE profiles of human and mouse samples.人类和小鼠样本的 FANTOM5 CAGE 图谱。
Sci Data. 2017 Aug 29;4:170112. doi: 10.1038/sdata.2017.112.