Lou Huihuang, Li Yi, Wu Yuejin, Hu Yuqin, Zhang Leyi
Wenzhou Center for Disease Control and Prevention, Wenzhou, China.
Foodborne Pathog Dis. 2025 Jun;22(6):414-421. doi: 10.1089/fpd.2024.0030. Epub 2024 Nov 20.
The infection status and etiological analysis of spp. from foodborne diarrhea patients in Wenzhou were carried out to provide the etiological basis for healthy diet and clinical treatment. isolates ( = 41) collected from foodborne diarrhea patients were identified using the automatic bacteriologic analyzer and mass spectrometer. Species identification, multilocus sequence typing, prediction of virulence genes, and antimicrobial resistance genes were analyzed by the data of whole genome sequencing. The antibiotic resistance of these isolates was determined using miniaturization of the broth dilution susceptibility test. A total of 1829 stool samples of diarrhea patients were collected, and the detection rate of spp. was 2.24% (41/1829). Moreover, spp. are more easily detected in warmer months (from June to August), which were identified as follows: (53.66%, 22/41), (21.95%, 9/41), (9.76%, 4/41), (4.88%, 2/41), (4.88%, 2/41), (2.44%, 1/41), and (2.44%, 1/41). All strains can be divided into 38 sequence types, 31 of which were novel, suggesting that spp. had high genetic diversity, multiple clones, and various sources in diarrhea patients. High number of genetic diversity and resistance were found in the isolates. In addition, the category distribution of the virulence genes was significantly different among the seven species of . spp. had different degrees of resistance to antibiotics, and tetracycline was the most serious, with a resistance rate of 27%. What's more, for some antimicrobial classes antimicrobial resistance gene detection was highly correlated with phenotypic antimicrobial resistance patterns with an overall sensitivity of 93.3% and a specificity of 66.7%. The findings from this research highlighted the importance for development of prevention and control strategies to reduce the risk of foodborne diarrhea caused by spp.
对温州食源性腹泻患者的某菌属感染状况及病原学进行分析，为健康饮食和临床治疗提供病原学依据。使用自动细菌分析仪和质谱仪对从食源性腹泻患者中收集的41株该菌属分离株进行鉴定。通过全基因组测序数据分析菌种鉴定、多位点序列分型、毒力基因预测和抗菌耐药基因。采用肉汤稀释药敏试验微型化方法测定这些分离株的抗生素耐药性。共收集了1829例腹泻患者的粪便样本，该菌属的检出率为2.24%（41/1829）。此外，该菌属在温暖月份（6月至8月）更容易检出，鉴定结果如下：某菌（53.66%，22/41）、某菌（21.95%，9/41）、某菌（9.76%，4/41）、某菌（4.88%，2/41）、某菌（4.88%，2/41）、某菌（2.44%，1/41）和某菌（2.44%，1/41）。所有菌株可分为38个序列型，其中31个为新序列型，表明该菌属在腹泻患者中具有高遗传多样性、多个克隆和多种来源。该菌属分离株中发现了大量的遗传多样性和耐药性。此外，该菌属的7个菌种中毒力基因的类别分布存在显著差异。该菌属对不同抗生素有不同程度的耐药性，对四环素耐药最为严重，耐药率为27%。此外，对于某些抗菌类别，抗菌耐药基因检测与表型抗菌耐药模式高度相关，总体敏感性为93.3%，特异性为66.7%。本研究结果突出了制定预防和控制策略以降低该菌属引起食源性腹泻风险的重要性。
