Zhao Baoyuan, Wang Yajuan, Wang Shengyuan, Mu Guangqing, Wu Xiaomeng
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China.
Science Center for Future Foods, Jiangnan University, Wuxi, China.
J Sci Food Agric. 2025 Mar 15;105(4):2594-2606. doi: 10.1002/jsfa.14032. Epub 2024 Nov 20.
Consumers require fermented milk that possesses constipation-relieving functions. To cater to the 'natural and additive-free' consumption habit, this study is dedicated to developing probiotic fermented milk with constipation-relief effects. Previously, we isolated two Lactobacillus strains, Lactobacillus MWLp-12 and Lactobacillus MWLf-4, from breast milk. This study evaluated the efficacy of these strains and their fermented milk in alleviating constipation in a Balb/c mouse model of constipation. The evaluation criteria included fecal water content, time for first black feces expulsion and propulsion rate of the small intestine. The mechanisms of constipation relief were investigated using gastrointestinal regulatory peptides, colonic tissue pathology, short-chain fatty acid levels and gut microbiota analyses.
Fecal water content, time for first black feces expulsion and small intestine propulsion rate indicated that both MWLp-12 and MWLf-4, as well as their fermented milk, could alleviate constipation in mice. Fermented milk exhibited superior effectiveness for relieving constipation compared to that of the strains alone. The results related to gastrointestinal regulatory peptides and short-chain fatty acids suggest that the mechanisms of constipation relief by the strains and their fermented milk may involve increased levels of 5-hydroxytryptamine and substance P in the mouse serum, higher concentrations of short-chain fatty acids in the intestines and decreased vasoactive intestinal peptide levels in the serum.
MWLp-12, MWLf-4 and fermented milk relieve constipation in mice. © 2024 Society of Chemical Industry.
消费者需要具有缓解便秘功能的发酵乳。为了迎合“天然且无添加剂”的消费习惯，本研究致力于开发具有缓解便秘作用的益生菌发酵乳。此前，我们从母乳中分离出两株乳酸菌，即MWLp - 12乳酸菌和MWLf - 4乳酸菌。本研究评估了这些菌株及其发酵乳在缓解便秘的Balb/c小鼠模型中便秘的效果。评估标准包括粪便含水量、首次排出黑色粪便的时间和小肠推进率。使用胃肠调节肽、结肠组织病理学、短链脂肪酸水平和肠道微生物群分析来研究便秘缓解的机制。
粪便含水量、首次排出黑色粪便的时间和小肠推进率表明，MWLp - 12和MWLf - 4及其发酵乳均可缓解小鼠便秘。与单独的菌株相比，发酵乳在缓解便秘方面表现出更优的效果。与胃肠调节肽和短链脂肪酸相关的结果表明，这些菌株及其发酵乳缓解便秘的机制可能涉及小鼠血清中5 - 羟色胺和P物质水平的升高、肠道中短链脂肪酸浓度的增加以及血清中血管活性肠肽水平的降低。
MWLp - 12、MWLf - 4和发酵乳可缓解小鼠便秘。© 2024化学工业协会。
