Transfection of 12/15-lipoxygenase effectively alleviates inflammatory responses during experimental acute pancreatitis.

BACKGROUND

Acute pancreatitis (AP), the initially triggered inflammatory process in the pancreas, can be life-threatening. It has been reported that 15-lipoxygenase may promote the removal of damaged intracellular components, maintain intracellular homeostasis, and promote apoptosis by upregulating the activity of caspases. Despite an increased understanding of the lipoxygenase pathway in inflammation and immune diseases, the role of the gene product in modulating the inflammatory changes during AP is not well defined.

AIM

To investigate the effect of expression in cerulein-induced AP in rats.

METHODS

Model rats were transfected with by injecting a recombinant lentivirus vector encoding into the left gastric artery before inducing AP. The expression of was then assessed at the mRNA and protein levels.

RESULTS

Our results showed that serum amylase activity and pancreatic tissue water content were significantly reduced in -transfected rats. Further, the mRNA expression levels of tumor necrosis factor alpha, interleukin (IL)-1β, IL-6, and monocyte chemoattractant protein-1, as well as the protein expression of nuclear factor kappa B in pancreatic tissue were reduced. Additionally, we observed an upregulation of cleaved caspase-3 that implies an induction of apoptosis in pancreatic cells. The transfection of resulted in a lower number of autophagic vacuoles in AP.

CONCLUSION

Our findings demonstrate a regulatory role of in apoptosis and autophagy, making it a potential therapeutic target for AP.