Yang Da Hee, Kim Hyo Jin, Dinh Duong Thi Thuy, Yang Jiwon, Hyun Chang-Lim, Jee Youngheun, Lee Naeun, Shin Min Sun, Kang Insoo, Kang Ki Soo
Graduate School, Jeju National University College of Medicine, Jeju, Korea.
Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, Korea.
Pediatr Gastroenterol Hepatol Nutr. 2024 Nov;27(6):345-354. doi: 10.5223/pghn.2024.27.6.345. Epub 2024 Nov 5.
The study aimed to investigate the recruiting of T lymphocytes including IL-7Rα CX3CR1 effector memory (EM) CD8 T cells and α4β7 integrin tagged T cells to inflamed intestinal mucosa.
Whole blood and mucosal tissues of intestine were collected from 40 children with or without inflammatory bowel disease (IBD). T cell surface staining and immunohistochemistry were done with several antibodies in peripheral blood mononuclear cells (PBMCs) and intestinal mucosa, respectively. Serum levels of cytokines were measured by ELISA.
The frequency of IL-7RαCX3CR1 EM CD8 T cells in the PBMC was significantly higher in the ulcerative colitis group than in the control group (57.9±17.80% vs. 33.9±15.70%, =0.021). The frequency of integrin α4β7 CD4 T cells in the PBMC was significantly lower in the ulcerative colitis group than in the control group (53.2±27.6% vs. 63.9±13.2%, =0.022). Serum concentration of TNF-α was higher in the Crohn's disease group than in the control group (26.13±5.01 pg/mL vs. 19.65±6.07 pg/mL, =0.008). Of the three groups, the ulcerative colitis group had the highest frequency of integrin α4β7 T cells based on immunohistochemistry analyses for intestinal tissues, followed by the Crohn's disease group and the control group (4.63±1.29 cells vs. 2.0±0.57 cells vs. 0.84±0.52 cells, <0.001).
Trafficking immune cells with effector memory CD8 T cells clarified by IL-7RαCX3CR1 and integrin α4β7 CD4 T cells might be highly associated with the pathogenesis of ulcerative colitis.
本研究旨在调查包括白细胞介素-7受体α（IL-7Rα）、CX3C趋化因子受体1（CX3CR1）效应记忆（EM）CD8 T细胞和α4β7整合素标记的T细胞在内的T淋巴细胞向炎症性肠黏膜的募集情况。
从40例患有或未患有炎症性肠病（IBD）的儿童中采集全血和肠黏膜组织。分别在外周血单个核细胞（PBMC）和肠黏膜中使用几种抗体进行T细胞表面染色和免疫组织化学检测。通过酶联免疫吸附测定（ELISA）测量细胞因子的血清水平。
溃疡性结肠炎组外周血单个核细胞中IL-7RαCX3CR1 EM CD8 T细胞的频率显著高于对照组（57.9±17.80%对33.9±15.70%，P = 0.021）。溃疡性结肠炎组外周血单个核细胞中整合素α4β7 CD4 T细胞的频率显著低于对照组（53.2±27.6%对63.9±13.2%，P = 0.022）。克罗恩病组的血清肿瘤坏死因子-α（TNF-α）浓度高于对照组（26.13±5.01 pg/mL对19.65±6.07 pg/mL，P = 0.008）。基于对肠组织的免疫组织化学分析，三组中溃疡性结肠炎组整合素α4β7 T细胞的频率最高，其次是克罗恩病组和对照组（4.63±1.29个细胞对2.0±0.57个细胞对0.84±0.52个细胞，P<0.001）。
由IL-7RαCX3CR1和整合素α4β7 CD4 T细胞所明确的具有效应记忆CD8 T细胞的免疫细胞转运可能与溃疡性结肠炎的发病机制高度相关。
