School of Science, Monash University Malaysia, Bandar Sunway, Selangor Darul Ehsan, Malaysia.
Microb Genom. 2024 Nov;10(11). doi: 10.1099/mgen.0.001327.
Despite being a major human pathogen, limited studies have reported RNA modifications in . These post-transcriptional modifications play crucial regulatory roles in bacteria and have also been shown to modulate bacterial virulence. Using nanopore sequencing, we characterized RNA modifications in a virulent strain (Ab-C98) under free-living (mid-exponential phase culture) and during an early stage of infection (3 h post-infection) in larvae. Analysis revealed that mC methylations are essential for ribosome synthesis, while mA and Ψ are involved in metabolic pathways and translation processes. Iron-chelating genes (mC and mA) and (mA and Ψ) and RNA polymerase subunit (mA and Ψ) were selectively modified during infection. This first transcriptome-wide study highlights the potential regulatory roles of mC, mA and Ψ modifications in during infection.
尽管是一种主要的人类病原体，但有限的研究报告了 在 RNA 修饰。这些转录后修饰在细菌中起着至关重要的调节作用，并且已经表明它们可以调节细菌的毒力。使用纳米孔测序，我们在自由生活（中指数期培养）和幼虫感染早期（感染后 3 小时）对一种毒力 的 菌株（Ab-C98）进行了 RNA 修饰的特征分析。分析表明，mC 甲基化对于核糖体合成是必不可少的，而 mA 和 Ψ 则参与代谢途径和翻译过程。感染过程中，铁螯合基因（mC 和 mA）和 （mA 和 Ψ）以及 RNA 聚合酶亚基（mA 和 Ψ）被选择性修饰。这是首次对 进行的全转录组研究，强调了 mC、mA 和 Ψ 修饰在感染过程中 在 中的潜在调节作用。
