Department of Cardiovascular Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan Province, China.
Department of Cardiology, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Clinical Medicine Research Center of Heart Failure of Hunan Province, Hunan Normal University, Changsha, China.
Sci Data. 2024 Nov 20;11(1):1257. doi: 10.1038/s41597-024-04115-0.
Pulmonary arterial hypertension (PAH) is a severe cardiopulmonary disease characterized by elevated pulmonary artery pressure and cardiac dysfunction, often leading to heart failure and even death. Despite the complexity of its mechanisms, the molecular basis of PAH remains unclear. tRNA-derived small RNAs (tsRNAs) are noncoding RNAs that play regulatory roles in various diseases. We collected plasma samples from PAH patients and healthy controls for small RNA microarray, and we established a monocrotaline-induced PAH rat model to collect right ventricular and lung tissues for tsRNA sequencing. Our analysis revealed 2,716 unique tsRNAs in human plasma and 4,733 in rat tissues, with a 7.84% overlap. Additionally, 204 tsRNAs were highly conserved across plasma, lung tissue, and right ventricle samples. The reproducibility of the expression profiles was confirmed through Pearson correlation and principal component analysis. KEGG pathway enrichment analysis indicated that tsRNAs are involved in key pathways, such as the MAPK and cancer signalling pathways. These datasets offer valuable insights for researching the epigenetic mechanisms underlying PAH and potential therapeutic targets.
肺动脉高压（PAH）是一种严重的心肺疾病，其特征是肺动脉压升高和心脏功能障碍，常导致心力衰竭甚至死亡。尽管其机制复杂，但 PAH 的分子基础仍不清楚。tRNA 衍生的小 RNA（tsRNA）是非编码 RNA，在各种疾病中发挥调节作用。我们收集了 PAH 患者和健康对照者的血浆样本进行小 RNA 微阵列分析，并建立了单硝酸异山梨酯诱导的 PAH 大鼠模型，以收集右心室和肺组织进行 tsRNA 测序。我们的分析在人类血浆中发现了 2716 个独特的 tsRNA，在大鼠组织中发现了 4733 个，有 7.84%的重叠。此外，204 个 tsRNA 在血浆、肺组织和右心室样本中高度保守。通过 Pearson 相关性和主成分分析证实了表达谱的重现性。KEGG 途径富集分析表明，tsRNA 参与了关键途径，如 MAPK 和癌症信号通路。这些数据集为研究 PAH 的表观遗传机制和潜在的治疗靶点提供了有价值的见解。
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