Key Laboratory of Genetic Evolution & Animal Models, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650201, P. R. China.
Yunnan Key Laboratory of Biodiversity Information, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650201, P. R. China.
Sci Data. 2024 Nov 20;11(1):1252. doi: 10.1038/s41597-024-04094-2.
The Spectacled Fulvetta (Fulvetta ruficapilla) is the type species of Fulvetta, an evolutionarily distinct group whose species show a high degree of sympatry in distribution and phenotypic convergence. To pave the way for insights into their adaptive evolution and speciation, we have assembled the first high quality reference genome for F. ruficapilla using high-fidelity (HiFi) long-read and Hi-C sequencing technologies. The resulting assembly spans a total of ~1.21 Gb with a contig N50 of 18.8 Mb and scaffold N50 of 75.9 Mb, and has a BUSCO completeness of 97.0%. The quality assessment suggests a high standard in base accuracy, continuity, and completeness of the assembly, comparable or close to that of Vertebrate Genomes Project. On this basis, we have annotated 23,774 protein-coding genes, of which 18,832 are functionally identified. The availability of this high-quality genome provides a solid foundation for the future studies of evolution and local adaptation in birds.
白脸鳾（Fulvetta ruficapilla）是鳾属的模式种，鳾属是一个进化上独特的类群，其物种在分布和表型趋同方面具有高度的同域性。为了深入了解它们的适应进化和物种形成，我们使用高保真（HiFi）长读长和 Hi-C 测序技术，为 F. ruficapilla 组装了第一个高质量的参考基因组。该组装总共跨越约 1.21Gb，具有 18.8Mb 的 contig N50 和 75.9Mb 的 scaffold N50，并且具有 97.0%的 BUSCO 完整性。质量评估表明，该组装在碱基准确性、连续性和完整性方面具有很高的标准，可与脊椎动物基因组计划相媲美或接近。在此基础上，我们注释了 23774 个编码蛋白的基因，其中 18832 个具有功能。这个高质量基因组的提供为鸟类进化和局部适应的未来研究奠定了坚实的基础。
