香港鲶鱼（Clarias fuscus）的改进染色体水平基因组组装与注释。

An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus).

作者信息

Chen Yu, Lin Xinghua, Zhou Dayan, Zhang Yulei, Wang Qian, Chen Huapu, Jiang Dongneng, Deng Siping, Li Guangli, Wang Peipei, Tian Changxu

机构信息

Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China.

Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, China.

出版信息

Sci Data. 2025 Feb 1;12(1):193. doi: 10.1038/s41597-025-04523-w.

DOI:10.1038/s41597-025-04523-w

PMID:39893196

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11787363/

Abstract

Clarias fuscus, renowned for its resilience and nutritional value, is a significant aquaculture species in China. To facilitate further genetic research and breeding programs in this species, we generated an improved high-quality chromosome-level genome assembly of a female C. fuscus using MGI, PacBio, and Hi-C sequencing technologies. The final genome assembly spans 982.84 Mb, with contig and scaffold N50 values of 36.16 Mb and 37.66 Mb, respectively, and successfully anchors 99.60% of the sequences to 28 pseudochromosomes. We also predicted 24,849 protein-coding genes, with 97.3% of them functionally annotated. BUSCO analysis indicates a completeness of 97.03% for the assembly and 96.6% for the annotation. This study significantly advances the genomic resources available for C. fuscus and supports future molecular breeding and functional genomics research.

摘要

胡子鲶以其适应力和营养价值而闻名，是中国重要的水产养殖品种。为了促进该物种的进一步遗传研究和育种计划，我们使用MGI、PacBio和Hi-C测序技术，对一条雌性胡子鲶进行了改进的高质量染色体水平基因组组装。最终的基因组组装大小为982.84 Mb，重叠群和支架的N50值分别为36.16 Mb和37.66 Mb，并成功将99.60%的序列锚定到28条假染色体上。我们还预测了24,849个蛋白质编码基因，其中97.3%的基因具有功能注释。BUSCO分析表明，该组装的完整性为97.03%，注释的完整性为96.6%。本研究显著推进了胡子鲶可用的基因组资源，并支持未来的分子育种和功能基因组学研究。