Liu Chang, Ren Lu, Yang Xiudong, Liu Yanqi, Zhang Huihong, Jia Wanru, Zhang Dawei
College of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin City 132022, PR China.
College of Aeronautical Engineering, Jilin Institute of Chemical Technology, Jilin City 132022, P R China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Mar 5;328:125436. doi: 10.1016/j.saa.2024.125436. Epub 2024 Nov 17.
A colorimetric fluorescent probe, 4-(1H-imidazolo[4,5-f][1,10]phenanthroline-2-yl)-N, N-diphenylaniline (PIN), was designed, synthesized and characterized for the sensitive and selective detection of Zn and Cd. The color of the solution changed from blue to yellow visible to the naked eye with the addition of Zn and Cd. The probe PIN showed good anti-interference to Zn and Cd in the presence of a variety of metal ions, and the fluorescence intensity showed a good linear relationship with the concentrations of Zn and Cd, with detection limits of 34.84 nM and 35.76 nM, respectively. The probe PIN complexed 2:1 with Zn and Cd, and the complexation constants were 1.03 × 10 M (PIN - Zn, R = 0.9971) and 1.50 × 10 M (PIN - Cd, R = 0.9981), respectively. In addition, the PIN could be recovered by EDTA and could be effectively monitored for Zn and Cd at pH 4-11, with good results in actual water samples. The HepG-2 cells maintained over 95 % of viability after 24 h exposure to PIN, which identified the extremely low toxic of PIN and could be used for in vivo cell imaging.
设计、合成并表征了一种比色荧光探针4-(1H-咪唑并[4,5-f][1,10]菲咯啉-2-基)-N,N-二苯胺（PIN），用于灵敏且选择性地检测锌和镉。加入锌和镉后，溶液颜色肉眼可见地从蓝色变为黄色。探针PIN在多种金属离子存在的情况下对锌和镉表现出良好的抗干扰性，并且荧光强度与锌和镉的浓度呈现良好的线性关系，检测限分别为34.84 nM和35.76 nM。探针PIN与锌和镉以2:1的比例络合，络合常数分别为1.03×10 M（PIN - Zn，R = 0.9971）和1.50×10 M（PIN - Cd，R = 0.9981）。此外，PIN可用乙二胺四乙酸回收，并且在pH 4 - 11范围内可有效监测锌和镉，在实际水样中效果良好。HepG-2细胞在暴露于PIN 24小时后存活率维持在95%以上，这表明PIN毒性极低，可用于体内细胞成像。
