Saleh Z M, Okeugo B, Venna V R, Blixt F W, Quaicoe V A, Park E S, Giorgberidze S, Luo M, Taylor C M, Rhoads J M, Liu Y
Division of Gastroenterology, Department of Pediatrics, McGovern Medical School, 12340The University of Texas Health Science Center at Houston, Houston, TX, USA.
BRAINS Research Laboratory, Department of Neurology, McGovern Medical School, 12340The University of Texas Health Science Center at Houston, Houston, TX, USA.
Benef Microbes. 2024 Nov 20;16(2):221-235. doi: 10.1163/18762891-bja00054.
Maternal separation (MS) in mice results in behavioral deficits and gut microbiota dysbiosis that all persist into adulthood. Limosilactobacillus reuteri DSM 17938 modulates gut microbiota, alters systemic metabolites, and facilitates immune regulation. To assess the effect of DSM 17938 on biochemical and behavioural stress-associated changes, newborn mice were exposed to unpredictable MS (MSU) daily from day 7 to day 20 of life, with intragastric administration of DSM 17938 or PBS as control. Body weight, brain levels of cholecystokinin (CCK), glial fibrillary acidic protein (GFAP), corticosterone, and stool microbiota were assessed at day 21. Behaviour tests including Y-maze (YMT), Tail Suspension (TST), and Open Field (OFT) were evaluated in adult mice. MSU resulted in a decrease in early postnatal growth, which improved with DSM 17938. Reduced CCK and increased corticosterone brain levels due to MSU were reversed by DSM 17938. GFAP levels increased with MSU, indicating that the decreased brain CCK was likely secondary to neuronal damage. DSM 17938 treated offspring demonstrated better cognitive function and less anxious behaviour in adult behaviour tests. DSM 17398 corrected stress related gut microbial dysbiosis. In conclusion, early life modulation of gut microbiota by DSM 17938 had beneficial effects on stress-associated physical and biochemical changes caused by MS in neonates and on subsequent adult behaviour.
小鼠的母婴分离（MS）会导致行为缺陷和肠道微生物群失调，且这些问题会持续到成年期。罗伊氏乳杆菌DSM 17938可调节肠道微生物群，改变全身代谢产物，并促进免疫调节。为了评估DSM 17938对与应激相关的生化和行为变化的影响，从出生后第7天到第20天，每天对新生小鼠进行不可预测的母婴分离（MSU），并通过胃内给药DSM 17938或PBS作为对照。在第21天评估体重、胆囊收缩素（CCK）、胶质纤维酸性蛋白（GFAP）、皮质酮的脑水平以及粪便微生物群。对成年小鼠进行包括Y迷宫（YMT）、悬尾试验（TST）和旷场试验（OFT）在内的行为测试。MSU导致出生后早期生长减缓，而DSM 17938可改善这一情况。DSM 17938可逆转因MSU导致的CCK降低和皮质酮脑水平升高。MSU使GFAP水平升高，表明脑内CCK降低可能继发于神经元损伤。经DSM 17938处理的后代在成年行为测试中表现出更好的认知功能和更少的焦虑行为。DSM 17398纠正了与应激相关的肠道微生物群失调。总之，DSM 17938对肠道微生物群的早期调节对新生儿期MS引起的应激相关生理和生化变化以及随后的成年行为具有有益影响。
