Wever J
Food Chem Toxicol. 1986 Mar;24(3):201-5. doi: 10.1016/0278-6915(86)90229-2.
Sulphite interacts reversibly and irreversibly in vitro with many compounds of biological relevance. A possible inhibitory action of sulphite on hepatic lactate dehydrogenase activity was studied using the perfused rat liver to determine glycolytic metabolites released by the liver during sulphite infusion. A sulphite-dependent reversible inhibition of lactate release but no increase in pyruvate production indicated that lactate dehydrogenase was inhibited by sulphite concentrations in the millimolar range. This effect was diminished by addition of pyruvate to the perfusion medium. It appears very unlikely that, under physiological conditions, sulphite levels in the portal blood or the liver of the rat would reach concentrations that inhibit lactate dehydrogenase activity.
亚硫酸盐在体外与许多具有生物学相关性的化合物发生可逆和不可逆的相互作用。使用灌注大鼠肝脏研究了亚硫酸盐对肝乳酸脱氢酶活性可能的抑制作用，以确定在输注亚硫酸盐期间肝脏释放的糖酵解代谢产物。亚硫酸盐依赖性地可逆抑制乳酸释放，但丙酮酸生成没有增加，这表明乳酸脱氢酶受到毫摩尔范围内亚硫酸盐浓度的抑制。向灌注介质中添加丙酮酸可减弱这种作用。在生理条件下，大鼠门静脉血或肝脏中的亚硫酸盐水平似乎极不可能达到抑制乳酸脱氢酶活性的浓度。
