State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Commun Biol. 2024 Nov 21;7(1):1549. doi: 10.1038/s42003-024-07259-2.
The recently identified novel duck reovirus (NDRV) is a waterfowl reovirus that can seriously harm or kill various waterfowl species. However, how NDRV interacts with host cells in Muscovy ducklings beyond the typical pathogenesis resulting from a viral infection is unknown. The current study examined the global translation efficiency of the Fabricius bursa of Muscovy ducklings infected with NDRV HN10 using mass spectrometry and ribosome footprint sequencing. Protein-protein interactions were investigated using immunogold labeling, transmission electron microscopy, and immunocytochemistry. An analysis of the relationship between mA and translation was performed using RNA immunoprecipitation and mA methylation immunoprecipitation. We found that both in vivo and in vitro, the translation efficiency of RNA modified with mA could be significantly reduced by σB, a structural protein component of NDRV HN10. Furthermore, σB might simultaneously interact with the stress granule complex CAPRIN1 and G3BP1 and the mA reader protein YTHDF1/3. Significant overlap was observed between m6A-modified and G3BP1-enriched RNA, indicating that granule stress could capture m6A-methylated RNA. We discovered a new function for NDRV HN10 in translational shutoff by recruiting mA-modified RNA into stress granules located in the Fabricius bursa of Muscovy ducklings.
最近发现的新型鸭呼肠孤病毒（NDRV）是一种水禽呼肠孤病毒，可严重危害或杀死各种水禽。然而，NDRV 如何与除了典型病毒感染引起的发病机制之外的番鸭雏鸭细胞相互作用尚不清楚。本研究使用质谱和核糖体足迹测序技术检测了感染 NDRV HN10 的番鸭法氏囊的整体翻译效率。使用免疫金标记、透射电子显微镜和免疫细胞化学研究了蛋白质-蛋白质相互作用。使用 RNA 免疫沉淀和 mA 甲基化免疫沉淀分析 mA 和翻译之间的关系。我们发现，无论是在体内还是体外，NDRV HN10 的结构蛋白成分 σB 都可以显著降低 mA 修饰的 RNA 的翻译效率。此外，σB 可能同时与应激颗粒复合物 CAPRIN1 和 G3BP1 以及 mA 读码蛋白 YTHDF1/3 相互作用。m6A 修饰和富含 G3BP1 的 RNA 之间存在显著重叠，表明颗粒应激可以捕获 m6A 甲基化 RNA。我们发现 NDRV HN10 通过将 mA 修饰的 RNA 募集到番鸭法氏囊中应激颗粒中，从而在翻译关闭中具有新的功能。
