Yang Huihu, Zhang Wandi, Wang Meihong, Yuan Sheng, Zhang Xuelian, Wen Feng, Guo Jinyue, Mei Kun, Huang Shujian, Li Zhili
College of Life Science and Engineering, Foshan University, Foshan, Guangdong, China.
Nanyang Vocational College of Agriculture, Nanyang, China.
Front Vet Sci. 2023 Mar 14;10:1124999. doi: 10.3389/fvets.2023.1124999. eCollection 2023.
The novel duck reovirus (NDRV) emerged in southeast China in 2005. The virus causes severe liver and spleen hemorrhage and necrosis in various duck species, bringing serious harm to waterfowl farming. In this study, three strains of NDRV designated as NDRV-ZSS-FJ20, NDRV-LRS-GD20, and NDRV-FJ19 were isolated from diseased Muscovy ducks in Guangdong and Fujian provinces. Pairwise sequence comparisons revealed that the three strains were closely related to NDRV, with nucleotide sequence identities for 10 genomic fragments ranging between 84.8 and 99.8%. In contrast, the nucleotide sequences of the three strains were only 38.9-80.9% similar to the chicken-origin reovirus and only 37.6-98.9% similar to the classical waterfowl-origin reovirus. Similarly, phylogenetic analysis revealed that the three strains clustered together with NDRV and were significantly different from classical waterfowl-origin reovirus and chicken-origin reovirus. In addition, the analyses showed that the L1 segment of the NDRV-FJ19 strain was a recombinant of 03G and J18 strains. Experimental reproduction of the disease showed that the NDRV-FJ19 strain was pathogenic to both ducks and chickens and could lead to symptoms of hemorrhage and necrosis in the liver and spleen. This was somewhat different from previous reports that NDRV is less pathogenic to chickens. In conclusion, we speculated that the NDRV-FJ19 causing duck liver and spleen necrosis is a new variant of a duck orthoreovirus that is significantly different in pathogenicity from any previously reported waterfowl-origin orthoreovirus.
新型鸭呼肠孤病毒（NDRV）于2005年在中国东南部出现。该病毒可导致多种鸭类出现严重的肝脏和脾脏出血及坏死，给水禽养殖业带来严重危害。在本研究中，从广东和福建患病番鸭中分离出三株NDRV，分别命名为NDRV-ZSS-FJ20、NDRV-LRS-GD20和NDRV-FJ19。两两序列比较显示，这三株病毒与NDRV密切相关，10个基因组片段的核苷酸序列同一性在84.8%至99.8%之间。相比之下，这三株病毒的核苷酸序列与鸡源呼肠孤病毒的相似性仅为38.9% - 80.9%，与经典水禽源呼肠孤病毒的相似性仅为37.6% - 98. .9%。同样，系统发育分析表明，这三株病毒与NDRV聚集在一起，与经典水禽源呼肠孤病毒和鸡源呼肠孤病毒有显著差异。此外，分析表明NDRV-FJ19株的L1片段是03G株和J18株的重组体。疾病的实验性再现表明，NDRV-FJ19株对鸭和鸡均具有致病性，可导致肝脏和脾脏出现出血和坏死症状。这与之前关于NDRV对鸡致病性较低的报道有所不同。总之，我们推测导致鸭肝脏和脾脏坏死的NDRV-FJ19是鸭正呼肠孤病毒的一个新变种，其致病性与之前报道的任何水禽源正呼肠孤病毒均有显著差异。
