Hunter-Chang Sarah, Kim-Aun Charlene, Karim Heeran, Jones Marieke, Vegiraju Tanvika, Stepanova Ekaterina, Manke Brynn, Kucenas Sarah, Deppmann Christopher
Department of Biology, University of Virginia, 485 McCormick Road, P.O. Box 400328, Charlottesville, VA, 22904-4328, USA.
Department of Neuroscience, University of Virginia, 200 Jeanette Lancaster Way, Charlottesville, VA, 22904-4328, USA.
bioRxiv. 2024 Nov 8:2024.11.08.622649. doi: 10.1101/2024.11.08.622649.
Axonal spheroids are hallmark features of neurodegeneration, forming along degenerating axons and contributing to disease progression. Despite their ubiquity across degenerative etiologies, the dynamics of spheroid disappearance, as well as their interactions with glial cells, remain poorly understood. Here, using an zebrafish model of peripheral nerve injury, we identified several patterns of spheroid disappearance that are regulated by Schwann cells. These results describe spheroid dynamics across their lifetimes, establish a role for the extra-axonal environment in altering spheroid outcomes, and identify a cellular mechanism whereby spheroid fates are altered.
轴突球体是神经退行性变的标志性特征，沿着退化的轴突形成并促进疾病进展。尽管它们在各种退行性病因中普遍存在，但球体消失的动态过程以及它们与神经胶质细胞的相互作用仍知之甚少。在这里，我们使用斑马鱼外周神经损伤模型，确定了由施万细胞调节的几种球体消失模式。这些结果描述了球体在其生命周期中的动态变化，确立了轴突外环境在改变球体结局中的作用，并确定了一种改变球体命运的细胞机制。
